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1996年采自俄罗斯吉丹半岛的武尔克摇蚊(双翅目,摇蚊科)的核型与基因序列

Karyotype and gene sequence of Wülker, 1996 (Diptera, Chironomidae) from the Gydan Peninsula, Russia.

作者信息

Bolshakov Viktor V, Prokin Alexander A, Artemenko Sergey V

机构信息

Papanin Institute for Biology of Inland Waters Russian Academy of Sciences, Yaroslavl reg., Nekouz prov., Borok, 152742, Russia Papanin Institute for Biology of Inland Waters Russian Academy of Sciences Borok Russia.

Cherepovets State University, Lunacharski 5,Cherepovets, 162600, Vologda Oblast', Russia Cherepovets State University Cherepovets Russia.

出版信息

Comp Cytogenet. 2021 Dec 7;15(4):447-458. doi: 10.3897/CompCytogen.v15i4.73135. eCollection 2021.

Abstract

The karyotype features and gene sequence of Wülker, 1996 from the Gydan Peninsula are presented for the first time. Nine banding sequences were determined, eight of them hpiA2, hpiB1, hpiC1, hpiC2, hpiD1, hpiE1, hpiF3 and hpiG1 were previously known from European, Georgian (South Caucasus) and Siberian populations. One new banding sequence for , hpiB2, was found. The hpiA2 banding sequence was found in all individuals, and this is its second finding after the Georgian population (Karmokov 2019). The hpiF3 banding sequence was found only in the homozygous state. Additional B-chromosomes are absent. The genetic distances (K2P) between gene sequence from Gydan Peninsula and Norway are 1.1--1.3%, and Georgia - 1.8%, much lower than the commonly accepted threshold of 3% for species of genus Meigen, 1803. The phylogenetic tree for gene sequences estimated by Bayesian inference showed geographically determined clusters of Norway and Gydan and a separate lineage of the Georgian population of . The analysis of karyotype and gene sequences shows that the population of from the Gydan Peninsula has an intermediate position within the group between Georgian, Yakutian and Norwegian populations. The position of Fabricius, 1787 from Canada and Greenland on the phylogenetic tree is discussed.

摘要

首次呈现了1996年来自吉丹半岛的武尔克(Wülker)的核型特征和基因序列。确定了9个条带序列,其中8个(hpiA2、hpiB1、hpiC1、hpiC2、hpiD1、hpiE1、hpiF3和hpiG1)先前在欧洲、格鲁吉亚(南高加索)和西伯利亚种群中已知。发现了一个新的条带序列hpiB2。hpiA2条带序列在所有个体中均被发现,这是继格鲁吉亚种群(卡尔莫科夫,2019年)之后的第二次发现。hpiF3条带序列仅在纯合状态下被发现。不存在额外的B染色体。吉丹半岛与挪威的基因序列之间的遗传距离(K2P)为1.1% - 1.3%,与格鲁吉亚的为1.8%,远低于1803年梅根属(Meigen)物种普遍接受的3%阈值。通过贝叶斯推断估计的基因序列系统发育树显示了挪威和吉丹在地理上确定的聚类以及格鲁吉亚种群的一个单独谱系。核型和基因序列分析表明,来自吉丹半岛的该种群在格鲁吉亚、雅库特和挪威种群之间的该类群中处于中间位置。讨论了1787年来自加拿大和格陵兰的法布里修斯(Fabricius)在系统发育树上的位置。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/197d/8671704/7ed225a7fd1a/comparative_cytogenetics-15-447-g001.jpg

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