缺氧诱导的 Maspin 表达影响卵巢透明细胞癌的预后。
Hypoxia-induced Maspin Expression Affects the Prognosis of Ovarian Clear Cell Carcinoma.
机构信息
Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Republic of Korea.
Department of Pathology, Seoul National University College of Medicine, Seoul, Republic of Korea.
出版信息
In Vivo. 2022 Jan-Feb;36(1):212-220. doi: 10.21873/invivo.12693.
BACKGROUND/AIM: To investigate the role of the expression of hypoxia-related genes on the prognosis of ovarian clear cell carcinoma (OCCC).
MATERIALS AND METHODS
Basal mRNA levels of eight hypoxia-related genes were compared. Cell viability was assayed after treating ES-2 cells under hypoxic conditions. The mRNA and protein levels were evaluated after the induction of hypoxia and administration of increased doses of N-acetylcysteine (NAC). Finally, the prognostic role of their expression levels was evaluated in 61 patients with OCCC.
RESULTS
The mRNA and protein levels of maspin increased gradually with the induction of hypoxia. Maspin protein expression decreased after treatment with paclitaxel and NAC. High expression of maspin was related to poor progression-free and overall survival in patients with OCCC (adjusted hazard ratios, 3.97 and 7.47; 95% confidence intervals=1.34-11.81, and 1.98-28.13).
CONCLUSION
High expression of maspin induced by hypoxia might be associated with poor prognosis of OCCC.
背景/目的：研究缺氧相关基因表达对卵巢透明细胞癌（OCCC）预后的作用。
材料与方法
比较了 8 个缺氧相关基因的基础 mRNA 水平。在缺氧条件下处理 ES-2 细胞后，检测细胞活力。在诱导缺氧并给予高剂量 N-乙酰半胱氨酸（NAC）后，评估 mRNA 和蛋白水平。最后，在 61 例 OCCC 患者中评估其表达水平的预后作用。
结果
随着缺氧的诱导，maspin 的 mRNA 和蛋白水平逐渐增加。紫杉醇和 NAC 处理后，maspin 蛋白表达降低。OCCC 患者中 maspin 高表达与无进展生存期和总生存期不良相关（调整后的危险比分别为 3.97 和 7.47；95%置信区间为 1.34-11.81 和 1.98-28.13）。
结论
缺氧诱导的 maspin 高表达可能与 OCCC 的不良预后有关。
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