Department of Chemistry, Division of Biology (Microbiology), University College of Qunfudah, Umm Al-Qura University, Al-Qunfudah, Saudi Arabia.
Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig, Egypt.
Environ Sci Pollut Res Int. 2022 Apr;29(18):27283-27293. doi: 10.1007/s11356-021-17925-1. Epub 2022 Jan 3.
The considerable effect of enzymes on human health draws great attention to enzyme-based drugs (therapeutic enzymes), in recent times. L-asparaginase (ASNase) is a well-known therapeutic enzyme. It has varied applications and is a single molecule for the treatment of multiple diseases. This study tries to extract asparaginase from soybean debris (agricultural wastes) as a cheap plant source and compare this with microbial asparaginase as an agent in cancer chemotherapy. The asparaginase was extracted and purified from soybean debris (plant asparaginase) and Pseudomonas aeruginosa (microbial asparaginase), then the physiochemical characters were determined for the two enzymes, and the anticancer activity of plant and microbial asparaginase was determined against gastric cancer (CLS-145), pancreatic cancer (AsPC-1), colon cancer (HCT116), esophagus cancer (KYSE-410), liver cancer (HepG2), breast cancer (MCF-7), and cervical cancer (HELLA). The results showed that plant asparaginase was superior to microbial asparaginase in its physiochemical characters. Plant asparaginase showed higher stability and activity under the conditions of changing either the temperature or the pH; also plant asparaginase has a higher affinity to the asparagine than the microbial asparaginase; besides, this plant asparaginase did not show activity with glutamine as a substrate. The plant asparaginase showed higher anticancer activity than that of microbial asparaginase against all studied cancer cell lines. The present study introduces as the first time a comparative study between the plant and microbial asparaginase which proves that soybean debris asparaginase can be more efficient and safe than that of the microbial asparaginase as an anticancer agent.
近年来，酶对人类健康的巨大影响引起了人们对基于酶的药物（治疗性酶）的极大关注。L-天冬酰胺酶（ASNase）是一种著名的治疗性酶。它有多种用途，是治疗多种疾病的单一分子。本研究试图从大豆废渣（农业废物）中提取天冬酰胺酶作为廉价的植物来源，并将其与微生物天冬酰胺酶作为癌症化疗中的药物进行比较。从大豆废渣（植物天冬酰胺酶）和铜绿假单胞菌（微生物天冬酰胺酶）中提取和纯化了天冬酰胺酶，然后确定了两种酶的理化特性，并测定了植物和微生物天冬酰胺酶对胃癌（CLS-145）、胰腺癌（AsPC-1）、结肠癌（HCT116）、食管癌（KYSE-410）、肝癌（HepG2）、乳腺癌（MCF-7）和宫颈癌（Hella）的抗癌活性。结果表明，植物天冬酰胺酶在理化性质方面优于微生物天冬酰胺酶。植物天冬酰胺酶在改变温度或 pH 值的条件下表现出更高的稳定性和活性；此外，植物天冬酰胺酶对天冬酰胺的亲和力高于微生物天冬酰胺酶；此外，这种植物天冬酰胺酶不以谷氨酰胺作为底物表现出活性。植物天冬酰胺酶对所有研究的癌细胞系的抗癌活性均高于微生物天冬酰胺酶。本研究首次对植物和微生物天冬酰胺酶进行了比较研究，证明大豆废渣天冬酰胺酶作为抗癌剂比微生物天冬酰胺酶更有效、更安全。
