State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation, National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, xiangshan street, haidian district, China.
Collaborative Innovation Center of Sustainable Forestry in Southern China, Nanjing Forestry University, longpan street, xuanwu district, China.
Tree Physiol. 2022 Jun 9;42(6):1286-1295. doi: 10.1093/treephys/tpab177.
As a new epigenetic mark, DNA N6-adenine (6mA) methylation plays an important role in various biological processes and has been reported in many prokaryotic organisms in recent years. However, the distribution patterns and functions of DNA 6mA modification have been poorly studied in non-model crops. In this study, we observed that the methylation ratio of 6mA was about 0.016% in the sea buckthorn (Hippophae rhamnoides L.) genome using mass spectrometry. We first constructed a comprehensive 6mA landscape in sea buckthorn genome using nanopore sequencing at single-base resolution. Distribution analysis suggested that 6mA methylated sites were widely distributed in the sea buckthorn chromosomes, which were similar to those in Arabidopsis and rice. Furthermore, reduced 6mA DNA methylation is associated with different expression of genes related to the fruit-ripening process in sea buckthorn. Our results revealed that 6mA DNA modification could be considered an important epigenomic mark and contributes to the fruit ripening process in plants.
作为一种新的表观遗传标记，DNA N6-腺嘌呤（6mA）甲基化在各种生物过程中发挥着重要作用，近年来在许多原核生物中都有报道。然而，在非模式作物中，DNA 6mA 修饰的分布模式和功能研究甚少。在本研究中，我们使用质谱法观察到沙棘（Hippophae rhamnoides L.）基因组中的 6mA 甲基化比例约为 0.016%。我们首先使用纳米孔测序技术以单碱基分辨率构建了沙棘基因组的综合 6mA 图谱。分布分析表明，6mA 甲基化位点广泛分布在沙棘染色体上，与拟南芥和水稻的分布相似。此外，沙棘中与果实成熟过程相关的基因的 6mA DNA 甲基化水平降低与这些基因的不同表达有关。我们的结果表明，6mA DNA 修饰可以被认为是一种重要的表观遗传标记，有助于植物的果实成熟过程。
