盐诱导激酶 2（SIK2）与合并慢性血吸虫病的结直肠癌患者淋巴结转移的关系。
Relationship between salt-inducible kinase 2 (SIK2) and lymph node metastasis in colorectal cancer patients complicated with chronic schistosomiasis.
机构信息
3. Department of Pathology, Wuhu Second People's Hospital, Wuhu 241002, Anhui Province, China.
出版信息
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2021 Oct 25;50(5):607-613. doi: 10.3724/zdxbyxb-2021-0157.
To investigate the relationship between salt-inducible kinase 2 (SIK2) and lymph node metastasis in colorectal cancer patients complicated with chronic schistosomiasis. Tissue specimens were collected from 363 patients who were diagnosed as colorectal cancer by clinical and pathological examination in Wuhu Second People's Hospital from June 2015 to June 2020. Fifty-six patients were colorectal cancer complicated with schistosomiasis (CRC-S) and 307 patients were colorectal cancer not complicated with schistosomiasis (CRC-NS). The clinical and pathological data of the patients were analyzed to explore the relationship between chronic schistosomiasis and colorectal cancer. Immunohistochemistry and Western blotting were used to detect the distribution and expression of SIK2 in colorectal cancer specimens. The relationship between SIK2 and lymph node metastasis of CRC-S was analyzed. The rate of lymph node metastasis in CRC-S group was significantly higher than that in CRC-NS group (62.5% vs. 47.2%, <0.05). In CRC-S patients with lymph node metastasis, schistosome eggs were distributed mainly in tumor tissues (25/35, 71.4%), while in patients with CRC-S without lymph node metastasis, schistosome eggs were distributed mainly in paracancerous tissues (17/21, 81.0%) (14.243, <0.01). The SIK2 was mainly located in cytosol, and its expression in tumor tissues was higher than that in paracancerous tissues. Compared with CRC-NS patients, the expression of SIK2 in CRC-S patients was significantly increased; the expression of SIK2 in patients with lymph node metastasis was higher than that in patients without lymph node metastasis; and the expression of SIK2 in patients with schistosome eggs in cancer tissues was higher than that in patients with schistosome eggs in paracancerous tissues (all <0.01). Lymph node metastasis is more likely to be occurred in colorectal cancer patients with schistosomiasis, especially in those with schistosome eggs in tumor tissues. The expression of SIK2 may be correlated with chronic schistosomiasis, egg distribution and lymphatic metastasis.
目的
探讨盐诱导激酶 2（SIK2）与合并慢性血吸虫病的结直肠癌患者淋巴结转移的关系。
方法
收集 2015 年 6 月至 2020 年 6 月在芜湖市第二人民医院经临床和病理检查诊断为结直肠癌的 363 例患者的组织标本。56 例为合并血吸虫病的结直肠癌（CRC-S）患者，307 例为不合并血吸虫病的结直肠癌（CRC-NS）患者。分析患者的临床病理资料，探讨慢性血吸虫病与结直肠癌的关系。采用免疫组化和 Western blot 检测 SIK2 在结直肠癌标本中的分布和表达，分析 SIK2 与 CRC-S 淋巴结转移的关系。
结果
CRC-S 组的淋巴结转移率明显高于 CRC-NS 组（62.5%比 47.2%，<0.05）。在有淋巴结转移的 CRC-S 患者中，血吸虫卵主要分布在肿瘤组织（25/35，71.4%），而在无淋巴结转移的 CRC-S 患者中，血吸虫卵主要分布在癌旁组织（17/21，81.0%）（14.243，<0.01）。SIK2 主要位于细胞质中，其在肿瘤组织中的表达高于癌旁组织。与 CRC-NS 患者相比，CRC-S 患者的 SIK2 表达明显增加；有淋巴结转移的患者 SIK2 表达高于无淋巴结转移的患者；肿瘤组织中有血吸虫卵的患者 SIK2 表达高于癌旁组织中有血吸虫卵的患者（均<0.01）。
结论
合并血吸虫病的结直肠癌患者更易发生淋巴结转移，尤其是肿瘤组织中有血吸虫卵的患者。SIK2 的表达可能与慢性血吸虫病、卵分布和淋巴转移有关。
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