高效液相色谱法和热喷雾高效液相色谱-质谱联用技术在生物介质中2',3'-二脱氧腺苷及其代谢产物分析与鉴定中的应用。
Application of high-performance liquid chromatography and thermospray high-performance liquid chromatography-mass spectrometry to the analysis and identification of 2',3'-dideoxyadenosine and its metabolite in biological media.
作者信息
Blau P A, Hines J W, Voyksner R D
机构信息
Analytical and Chemical Sciences, Research Triangle Institute, NC 27709.
出版信息
J Chromatogr. 1987 Sep 4;420(1):1-12. doi: 10.1016/0378-4347(87)80149-4.
Reversed-phase high-performance liquid chromatographic (HPLC) procedures are described for determining the stability of 2',3'-dideoxyadenosine (DDA) in biological fluids at therapeutic dosages. The validated methodology uses both direct injection and solid-phase extraction techniques. Deamination of DDA to 2',3'-dideoxyinosine (DDI) in plasma by adenosine deaminase was monitored by HPLC, and the identification of DDI verified by thermospray HPLC-mass spectrometry. This methodology should prove useful in future studies concerning the stability and metabolism of dideoxynucleosides.
描述了反相高效液相色谱（HPLC）方法，用于测定治疗剂量下生物流体中2'，3'-二脱氧腺苷（DDA）的稳定性。经过验证的方法使用了直接进样和固相萃取技术。通过HPLC监测血浆中腺苷脱氨酶将DDA脱氨生成2'，3'-二脱氧肌苷（DDI）的过程，并通过热喷雾HPLC-质谱法对DDI进行鉴定。该方法在未来关于双脱氧核苷稳定性和代谢的研究中应会证明是有用的。
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