一种使用定量 MALDI 质谱法分析人血清中多种蛋白质生物标志物的新方法。
A novel method for multiplex protein biomarker analysis of human serum using quantitative MALDI mass spectrometry.
机构信息
Smorodintsev Research Institute of Influenza, div. Russian Ministry of Health, 15/17 Ulitsa Professora Popova, St. Petersburg 197376, Russia; Peter the Great St. Petersburg Polytechnic University, 29 Ulitsa Polytechnicheskaya, St. Petersburg 194064, Russia.
Peter the Great St. Petersburg Polytechnic University, 29 Ulitsa Polytechnicheskaya, St. Petersburg 194064, Russia.
出版信息
J Pharm Biomed Anal. 2022 Feb 20;210:114575. doi: 10.1016/j.jpba.2021.114575. Epub 2022 Jan 3.
In this work, we have extended our previously proposed approach for determining protein concentrations in human serum (using MALDI-TOF mass spectrometry) to include simultaneous analysis of several proteins associated with acute inflammation (alpha-2-macroglobulin, fetuin-A, serum amyloid A1). This technique can be used to diagnose systemic inflammation and provides results in 4-5 h. The developed approach was verified using standard immunological methods (ELISA). Samples from 87 individuals, in specific groups, were used for testing and validation: control; inflammatory soft tissue disease accompanied by sepsis; influenza A infection; or COVID-19. The feasibility of differentiating patient groups with the aforementioned conditions was analyzed using a combination of the inflammatory markers described. For fetuin-A and serum amyloid A1, diagnostically significant concentration ranges were established.
在这项工作中，我们扩展了之前提出的用于确定人血清中蛋白质浓度（使用 MALDI-TOF 质谱法）的方法，以包括同时分析几种与急性炎症相关的蛋白质（α-2-巨球蛋白、胎球蛋白-A、血清淀粉样蛋白 A1）。该技术可用于诊断全身炎症，并在 4-5 小时内提供结果。所开发的方法使用标准免疫学方法（ELISA）进行了验证。使用来自特定组别的 87 个人的样本进行了测试和验证：对照组；伴有败血症的炎症性软组织疾病；甲型流感感染；或 COVID-19。使用所描述的炎症标志物组合分析了区分具有上述情况的患者组的可行性。对于胎球蛋白-A 和血清淀粉样蛋白 A1，建立了具有诊断意义的浓度范围。
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