Ujiie T
Department of Experimental Therapeutics, Kanazawa University, Japan.
Jpn J Exp Med. 1987 Jun;57(3):183-8.
Spleen cells of untreated mice became nonspecifically cytotoxic after cultivation in the presence of a streptococcal preparation, OK-432, for 3 or more days. The cytotoxic cells, named OK-432-induced killer (OIK) cells, injured a variety of target cells including syngeneic EL-4 cells resistant to natural killer (NK) cells, but not NK-susceptible YAC-1 cells. C57BL/6 splenic cells cultured for 5 days in the presence of both OK-432 and interleukin 2 (IL-2) were highly cytotoxic to both EL-4 and YAC-1 cells, and had Thy-1+, Lyt-1,2-, and asialogGM1+ phenotypes, which were identical with those of OIK cells. A test for competitive inhibition with cold target cells and fractionation by centrifugation onto discontinuous density gradients of Percoll showed that cytotoxic cells generated by OK-432 plus IL-2 comprised at least two populations; the cells in the first population were cytotoxic to EL-4 cells but not to YAC-1 cells, and were smaller in size than those in the second population, which were cytotoxic to YAC-1 cells and had NK morphology. Therefore, generation of OIK cells was augmented by IL-2.
未处理小鼠的脾细胞在链球菌制剂OK-432存在下培养3天或更长时间后会变成非特异性细胞毒性细胞。这些细胞毒性细胞被命名为OK-432诱导杀伤（OIK）细胞，可损伤多种靶细胞，包括对自然杀伤（NK）细胞有抗性的同基因EL-4细胞，但不损伤对NK敏感的YAC-1细胞。在OK-432和白细胞介素2（IL-2）同时存在的情况下培养5天的C57BL/6脾细胞对EL-4和YAC-1细胞均具有高度细胞毒性，并且具有Thy-1+、Lyt-1,2-和去唾液酸GM1+表型，这些表型与OIK细胞相同。用冷靶细胞进行竞争抑制试验以及通过在不连续密度的Percoll梯度上离心进行分离表明，由OK-432加IL-2产生的细胞毒性细胞至少包括两个群体；第一个群体中的细胞对EL-4细胞具有细胞毒性，但对YAC-1细胞没有细胞毒性，并且其大小比第二个群体中的细胞小，第二个群体中的细胞对YAC-1细胞具有细胞毒性并且具有NK形态。因此，IL-2增强了OIK细胞的产生。
