Department of Radiology, The Second Affiliated Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong 510180, China.
Guangzhou Institute of Chemistry, Chinese Academy of Science, 510650 Guangzhou, China.
Can J Gastroenterol Hepatol. 2021 Dec 30;2021:2909189. doi: 10.1155/2021/2909189. eCollection 2021.
To investigate the treatment effect of a vascular-disrupting agent, M410, using diffusion-weighted imaging in a rabbit model of hepatic VX2 tumor.
28 New Zealand white rabbit models with VX2 liver tumors were established and were randomly divided into M410 (intravenous injection of M410 at a dose of 25 mg/kg every three days) and control (intravenous injection of saline every three days) groups. Conventional and diffusion-weighted imaging (DWI) were acquired on a 3.0 T MR unit at baseline, 4 h, d 1, d 4, d 7, and d 14 posttreatment. B-value with 700 (s/mm) was chosen during DWI examinations. Tumor volume and apparent diffusion coefficient (ADC) values of the entire tumor and solid component of the tumor at every time point were measured. Two randomly chosen rabbits from each group were sacrificed for H&E staining and CD34 immunohistochemical assessments at each time point. An independent sample -test was used to assess differences in tumor sizes and ADC values of the entire tumor and solid component of tumors between two groups, with < 0.05 considered statistically significant.
There was no significant difference in tumor volume between the two groups at baseline, 4 h, and d 1. With time, the tumors in the control group grew significantly faster than those in the M410 group, and the average ADC values of the M410 group were lower than those of the control group at d 1 and higher than those of the control group at d 4; as such, there were statistical differences between the two groups at these two time points but not at the other four time points. The following pathological results reflected the underlying morphological changes and vascular alterations.
M410 performed well in inhibiting the growth of the hepatic VX2 tumor which could be noninvasively monitored by DWI metrics.
在兔 VX2 肝肿瘤模型中，通过扩散加权成像（DWI）研究血管破坏剂 M410 的治疗效果。
建立 28 只新西兰白兔 VX2 肝肿瘤模型，随机分为 M410 组（静脉注射 M410，剂量为 25mg/kg，每 3 天 1 次）和对照组（每 3 天静脉注射生理盐水 1 次）。在基线、治疗后 4h、第 1、4、7 和 14 天，使用 3.0TMR 设备进行常规和 DWI 扫描。DWI 检查时选择 b 值为 700（s/mm）。测量每个时间点整个肿瘤和肿瘤实性部分的肿瘤体积和表观扩散系数（ADC）值。每组随机选择 2 只兔子，在每个时间点进行 H&E 染色和 CD34 免疫组化评估。采用独立样本 t 检验比较两组肿瘤体积和整个肿瘤及肿瘤实性部分 ADC 值的差异， < 0.05 为差异有统计学意义。
两组基线、4h 和第 1 天的肿瘤体积无显著差异。随着时间的推移，对照组肿瘤生长速度明显快于 M410 组，M410 组在第 1 天和第 4 天的平均 ADC 值低于对照组，而在第 4 天高于对照组，这两个时间点有统计学差异，而其他四个时间点没有统计学差异。以下病理结果反映了潜在的形态学变化和血管改变。
M410 可有效抑制兔 VX2 肝肿瘤的生长，DWI 指标可对其进行无创监测。
