Velu T J, Beguinot L, Vass W C, Willingham M C, Merlino G T, Pastan I, Lowy D R
Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, MD 20892.
Science. 1987 Dec 4;238(4832):1408-10. doi: 10.1126/science.3500513.
The epidermal growth factor (EGF) receptor gene EGFR has been placed in a retrovirus vector to examine the growth properties of cells that experimentally overproduce a full-length EGF receptor. NIH 3T3 cells transfected with the viral DNA or infected with the corresponding rescued retrovirus developed a fully transformed phenotype in vitro that required both functional EGFR expression and the presence of EGF in the growth medium. Cells expressing 4 x 10(5) EGF receptors formed tumors in nude mice, while control cells did not. Therefore, the EGFR retrovirus, which had a titer on NIH 3T3 cells that was greater than 10(7) focus-forming units per milliliter, can efficiently transfer and express this gene, and increased numbers of EGF receptors can contribute to the transformed phenotype.
表皮生长因子（EGF）受体基因EGFR已被置于逆转录病毒载体中，以研究实验性过量产生全长EGF受体的细胞的生长特性。用病毒DNA转染或用相应的拯救逆转录病毒感染的NIH 3T3细胞在体外形成了完全转化的表型，这既需要功能性EGFR表达，也需要生长培养基中存在EGF。表达4×10⁵个EGF受体的细胞在裸鼠中形成肿瘤，而对照细胞则不会。因此，在NIH 3T3细胞上滴度大于每毫升10⁷个集落形成单位的EGFR逆转录病毒能够有效地转移和表达该基因，并且增加的EGF受体数量可导致转化表型。
