一种新型的差分式迁移率谱串联质谱法，用于体内熊果酸的定量分析。
A novel, differential mobility spectrometry tandem mass spectrometric method for the in vivo quantitation of ursolic acid.
机构信息
Research Center for Drug Metabolism, School of Life Science, Jilin University, Changchun 130012, PR China; Beijing Institute of Drug Metabolism, Beijing 102209, PR China.
Research Center for Drug Metabolism, School of Life Science, Jilin University, Changchun 130012, PR China; Beijing Institute of Drug Metabolism, Beijing 102209, PR China.
出版信息
J Pharm Biomed Anal. 2022 Feb 20;210:114559. doi: 10.1016/j.jpba.2021.114559. Epub 2021 Dec 28.
Ursolic acid (UA) is a naturally occurring pentacyclic triterpene widely distributed in fruits and plants. It is pharmacologically active and has the potential to be a useful therapeutic compound. To date, bioanalysis of UA has been limited by biomatrix interference and poor collision induced dissociation (CID) efficiency in tandem mass spectrometry. In this study, we developed a method based on liquid chromatography differential mobility spectrometry tandem mass spectrometry LC-DMS-MS/MS with multiple ion monitoring (MIM) for quantitation of UA in rat plasma. The method involves efficient sample preparation by solid phase extraction and requires only a limited volume of plasma (40 μL) to achieve linearity in the 1-100 ng/mL range with good accuracy and precision. The method was successfully applied to a pharmacokinetic study of orally administered UA in rat. The results indicate that LC-DMS-MS/MS with MIM is a useful strategy for the bioassay of UA suitable for high throughput analysis.
熊果酸（UA）是一种广泛存在于水果和植物中的天然五环三萜。它具有药理学活性，有可能成为一种有用的治疗化合物。迄今为止，UA 的生物分析受到生物基质干扰和串联质谱中较差的碰撞诱导解离（CID）效率的限制。在这项研究中，我们开发了一种基于液相色谱差分迁移谱串联质谱 LC-DMS-MS/MS 与多离子监测（MIM）的方法，用于定量测定大鼠血浆中的 UA。该方法通过固相萃取进行有效的样品制备，仅需 40 μL 有限量的血浆即可在 1-100ng/mL 范围内实现线性，具有良好的准确性和精密度。该方法成功地应用于大鼠口服 UA 的药代动力学研究。结果表明，MIM 的 LC-DMS-MS/MS 是一种用于 UA 生物测定的有用策略，适合高通量分析。
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