刚果民主共和国金沙萨学龄儿童恶性疟原虫分离株中与耐药性相关基因多态性的鉴定。
Identification of polymorphisms in genes associated with drug resistance in Plasmodium falciparum isolates from school-age children in Kinshasa, Democratic Republic of Congo.
作者信息
Nundu Sabin S, Culleton Richard, Simpson Shirley V, Arima Hiroaki, Chitama Ben-Yeddy Abel, Muyembe Jean-Jacques, Ahuka Steve, Kaneko Osamu, Mita Toshihiro, Yamamoto Taro
机构信息
Program for Nurturing Global Leaders in Tropical and Emerging Communicable Diseases, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan; Department of International Health and Medical Anthropology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Institut National de Recherche Biomédicale, Kinshasa, Democratic Republic of the Congo.
Division of Molecular Parasitology, Proteo-Science Centre, Ehime University, Ehime, Japan; Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan.
出版信息
Parasitol Int. 2022 Jun;88:102541. doi: 10.1016/j.parint.2022.102541. Epub 2022 Jan 17.
BACKGROUND
The emergence and spread of Plasmodium falciparum parasites resistant to antimalarial drugs constitutes an obstacle to malaria control and elimination. This study aimed to identify the prevalence of polymorphisms in pfk13, pfmdr1, pfdhfr, pfdhps and pfcrt genes in isolates from asymptomatic and symptomatic school-age children in Kinshasa.
METHODS
Nested-PCR followed by sequencing was performed for the detection of pfk13, pfmdr1, pfdhfr, pfdhps and pfcrt polymorphisms.
RESULTS
Two mutations in pfk13, C532S and Q613E were identified in the Democratic Republic of Congo for the first time. The prevalence of the drug-resistance associated mutations pfcrt K76T, pfdhps K540E and pfmdr1 N86Y was low, being 27%, 20% and 9%, respectively.
CONCLUSION
We found a low prevalence of genetic markers associated with chloroquine and sulfadoxine-pyrimethamine resistance in Kinshasa. Furthermore, no mutations previously associated with resistance against artemisinin and its derivatives were observed in the pfK13 gene. These findings support the continued use of ACTs and IPTp-SP. Continuous molecular monitoring of antimalarial resistance markers is recommended.
背景
恶性疟原虫对抗疟药物的耐药性出现和传播构成了疟疾控制和消除的障碍。本研究旨在确定金沙萨无症状和有症状学龄儿童分离株中pfk13、pfmdr1、pfdhfr、pfdhps和pfcrt基因多态性的流行情况。
方法
采用巢式PCR随后测序来检测pfk13、pfmdr1、pfdhfr、pfdhps和pfcrt多态性。
结果
在刚果民主共和国首次鉴定出pfk13的两个突变，即C532S和Q613E。与耐药相关的突变pfcrt K76T、pfdhps K540E和pfmdr1 N86Y的流行率较低，分别为27%、20%和9%。
结论
我们发现金沙萨与氯喹和磺胺多辛-乙胺嘧啶耐药相关的遗传标记物流行率较低。此外，在pfK13基因中未观察到先前与青蒿素及其衍生物耐药相关的突变。这些发现支持继续使用青蒿素类复方疗法和孕期间歇性预防治疗-磺胺多辛-乙胺嘧啶。建议持续对抗疟药物耐药性标记物进行分子监测。
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