Hu Lin, Sun Zhongxiang, Xu Cuicui, Wang Jie, Mallik Azim U, Gu Chengzhen, Chen Daoqian, Lu Long, Zeng Rensen, Song Yuanyuan
Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Key Laboratory of Beibu Gulf Environment Change and Resources Utilization of Ministry of Education, Nanning Normal University, Nanning 530001, China.
iScience. 2022 Jan 2;25(1):103726. doi: 10.1016/j.isci.2021.103726. eCollection 2022 Jan 21.
Abuse of chemical fertilizers and insecticides has created many environmental and human health hazards. We hypothesized that high nitrogen (N) in crops changes insect gut microbiota leading to enhanced insecticide tolerance. We investigated the effect of high N in maize on gut microbiota and insecticide tolerance of the polyphagous pest . Bioassays showed that high N applied in both maize plants and artificial diets significantly enhanced larval growth but reduced larval sensitivity to the insecticide methomyl. High N promoted the gut bacterial abundance in the genus . Inoculation with two strains ( and ) isolated from the larval guts increased larval tolerance to methomyl. Incorporation of antibiotics in a high-N diet increased the larval sensitivity to methomyl. These findings suggest that excessive application of N fertilizer to crops can increase insecticide tolerance of insect pests via changing gut microbiota, leading to increased use of insecticides worldwide.
化肥和杀虫剂的滥用造成了许多环境和人类健康危害。我们推测，作物中高含量的氮会改变昆虫肠道微生物群，从而提高昆虫对杀虫剂的耐受性。我们研究了玉米中高氮对多食性害虫肠道微生物群和杀虫剂耐受性的影响。生物测定表明，在玉米植株和人工饲料中施用高氮均显著促进幼虫生长，但降低了幼虫对杀虫剂灭多威的敏感性。高氮促进了该属肠道细菌的丰度。接种从幼虫肠道分离出的两种菌株（ 和 ）可提高幼虫对灭多威的耐受性。在高氮饲料中添加抗生素会增加幼虫对灭多威的敏感性。这些发现表明，向作物过量施用氮肥会通过改变肠道微生物群来提高害虫对杀虫剂的耐受性，从而导致全球范围内杀虫剂使用量增加。
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