羊膜同种异体移植治疗实验性牙龈退缩缺损的愈合：犬的组织学和组织计量学分析

Healing of experimental gingival recession defects treated with amnion allograft: Histologic and histometric analysis in dogs.

作者信息

Gomaa Marwa, El Guindy Hoda, Shoukheba Malak, Metwalli Alaa

机构信息

Department of Oral Medicine, Periodontology, Oral Diagnosis and Oral Radiology, Faculty of Dentistry, Tanta University, Tanta, Egypt.

出版信息

J Oral Biosci. 2022 Mar;64(1):93-99. doi: 10.1016/j.job.2022.01.006. Epub 2022 Jan 22.

DOI:10.1016/j.job.2022.01.006

PMID:35077863

Abstract

OBJECTIVES

This study was conducted to compare the healing response of localized gingival recession defects treated with a coronally advanced flap (CAF) and either an amnion allograft membrane (AM) or a connective tissue graft (CTG).

METHODS

Gingival recession defects were surgically created in six healthy mongrel dogs at the labial root surface of the maxillary canines, bilaterally. Using a split mouth design, the defects were treated with CAF and either AM (CAF/AM) or CTG (CAF/CTG). Three animals for each group were scarified at 1 and 3 months. Segments containing the defects were prepared for histological and histometric analysis.

RESULTS

Both techniques showed similar clinical findings with adequate root coverage. Histologically, healing was characterized by the formation of new cementum and new connective tissue attachment in the CAF/AM group; in the CAF/CTG group, healing was characterized by junctional epithelium, coronally, and connective tissue fibers parallel to the root surface, apically. Histometrically, the CAF/AM group revealed a substantially shorter epithelial length and a longer, new cementum compared with those of the CAF/CTG group after a healing period of 3 months.

CONCLUSIONS

Within the limits of this study, we concluded that the AM allograft could promote periodontal healing in gingival recession defects.

摘要

目的

本研究旨在比较用冠向复位瓣（CAF）联合羊膜同种异体移植膜（AM）或结缔组织移植（CTG）治疗局限性牙龈退缩缺损的愈合反应。

方法

在6只健康杂种犬双侧上颌尖牙唇侧牙根表面手术制造牙龈退缩缺损。采用双侧对照设计，缺损分别用CAF联合AM（CAF/AM）或CAF联合CTG（CAF/CTG）治疗。每组3只动物分别在1个月和3个月时处死。制备含缺损的组织块用于组织学和组织计量学分析。

结果

两种技术均显示出相似的临床结果，牙根覆盖良好。组织学上，CAF/AM组愈合的特征是形成新的牙骨质和新的结缔组织附着；在CAF/CTG组，愈合的特征是冠方为结合上皮，根尖方为平行于牙根表面的结缔组织纤维。组织计量学分析显示，愈合3个月后，CAF/AM组的上皮长度明显短于CAF/CTG组，新形成的牙骨质更长。