利用激光烧蚀-同位素比质谱（LA-IRMS）和基质辅助激光解吸/电离成像质谱（MALDI-IMS）可视化富勒醇纳米颗粒在大型溞中的分布。

Visualization of fullerenol nanoparticles distribution in Daphnia magna using Laser Ablation-isotope Ratio Mass (LA-IRMS) and Matrix-assisted Laser Desorption/Ionization Imaging Mass Spectrometry (MALDI-IMS).

机构信息

Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; National Engineering Laboratory for Lake Pollution Control and Ecological Restoration, Beijing 100012, China; State Environmental Protection Key Laboratory for Lake Pollution Control, Beijing 100012, China; State Environmental Protection Scientific Observation and Research Station for Lake Dongtinghu, Beijing 100012, China; State Environmental Protection Key Laboratory of Estuarine and Coastal Environment, Beijing 100012, China; Chinese Research Academy of Environmental Sciences, Beijing 100012, China.

Global Centre for Environmental Remediation, University of Newcastle, Callaghan, NSW 2308, Australia; Cooperative Research Centre for Contamination Assessment and Remediation of the Environment, University of Newcastle, Callaghan, NSW 2308, Australia.

出版信息

Ecotoxicol Environ Saf. 2022 Mar 1;232:113226. doi: 10.1016/j.ecoenv.2022.113226. Epub 2022 Jan 29.

DOI:10.1016/j.ecoenv.2022.113226

PMID:35093811

Abstract

Laser ablation-isotope ratio mass spectrometry (LA-IRMS) allows the mapping analysis of carbon isotope (δC) signature in organism samples.Matrix assisted laser desorption ionization time-of-flightimaging mass spectrometry (MALDI-TOF-IMS) enables image of target directly. In this study, the distribution of δC and fullerenol nanoparticles in Daphnia magna (D. magna) exposed to different fullerenol solution are mapped using the LA-IRSM and MALDI-TOF-IMS for comparison. We visualize thedistribution of fullerenol nanoparticles mainly in the intestine, also in other parts of the body as well. This is the first time that fullerenol nanoparticles was found outside the intestine of D. magna, which has been confirmed by the two imaging methods individually. Although the both imaging methods are applicable to in-situ visualize the localization and spatial distribution of fullerenol nanoparticles in organisms, MALDI-TOF-IMS is more suitable, in terms of sample preparation and image resolution. The results of our study will also provide a new idea and method for the research of environmental toxicology.

摘要

激光烧蚀-同位素比质谱（LA-IRMS）允许对生物体样本中的碳同位素（δC）特征进行映射分析。基质辅助激光解吸电离飞行时间成像质谱（MALDI-TOF-IMS）能够直接对目标进行成像。在这项研究中，使用 LA-IRSM 和 MALDI-TOF-IMS 对暴露于不同富勒醇溶液中的大型溞（Daphnia magna，D. magna）中的 δC 和富勒醇纳米颗粒的分布进行了绘图比较。我们观察到富勒醇纳米颗粒主要分布在肠道中，也分布在身体的其他部位。这是首次在大型溞的肠道外发现富勒醇纳米颗粒，这两种成像方法都分别证实了这一点。尽管两种成像方法都适用于原位可视化生物体中富勒醇纳米颗粒的定位和空间分布，但 MALDI-TOF-IMS 在样品制备和图像分辨率方面更适用。我们的研究结果还将为环境毒理学研究提供新的思路和方法。