Sherwood N M, Harvey B
Gen Comp Endocrinol. 1986 Jan;61(1):13-9. doi: 10.1016/0016-6480(86)90244-3.
The studies reported here show that exogenous GnRH can be absorbed by goldfish from the intraperitoneal (ip) cavity, gill surface, or surrounding water. Mammalian rather than teleost GnRH was applied in order to ensure that GnRH measurement in plasma did not reflect the native form. A radioimmunoassay (RIA) specific to mammalian GnRH was used to measure the concentration of absorbed GnRH; validation for this approach was provided by HPLC and cross-reactivity studies in which mammalian GnRH was shown not to be present in control goldfish brain or pituitary extracts. Plasma concentration of GnRH was highest at the first sampling time, 4 min after administration, for all three routes. For intraperitoneal injection, plasma concentration was halved in 12 min, a period comparable with the half-life in rats. The pituitary content of GnRH also increased rapidly during the first 4 min after ip injection and remained high for 60 min. Absorption of GnRH from the gill was equally effective with water or dimethyl sulfoxide (DMSO) as vehicle.
此处报告的研究表明，外源促性腺激素释放激素（GnRH）可被金鱼从腹腔、鳃表面或周围水体吸收。为确保血浆中GnRH的测量不反映其天然形式，使用的是哺乳动物而非硬骨鱼的GnRH。采用针对哺乳动物GnRH的放射免疫分析法（RIA）来测量吸收的GnRH浓度；高效液相色谱法（HPLC）和交叉反应性研究为该方法提供了验证，研究表明对照金鱼的脑或垂体提取物中不存在哺乳动物GnRH。对于所有三种给药途径，在给药后4分钟的首次采样时间，血浆中GnRH的浓度最高。对于腹腔注射，血浆浓度在12分钟内减半，这一时间段与大鼠体内的半衰期相当。腹腔注射后最初4分钟内，垂体中GnRH的含量也迅速增加，并在60分钟内保持在较高水平。以水或二甲基亚砜（DMSO）作为载体时，GnRH从鳃的吸收效果相同。
