CUBIC 法用于胚胎和成年小鼠全器官 X-gal 染色与组织透明化的方案

Protocol for whole-mount X-gal staining combined with tissue clearing in embryo and adult mouse using CUBIC.

机构信息

Department of Cell Biology, National Cerebral and Cardiovascular Center Research Institute, 6-1 Kishibe-shimmachi, Suita, Osaka 564-8565, Japan.

Department of Molecular Pathophysiology, Institute of Advanced Medical Sciences, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan.

出版信息

STAR Protoc. 2022 Jan 20;3(1):101127. doi: 10.1016/j.xpro.2022.101127. eCollection 2022 Mar 18.

DOI:10.1016/j.xpro.2022.101127

PMID:35118431

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8792446/

Abstract

Here we describe an optimized protocol for X-gal staining of tissue clearing embryo and adult mouse using CUBIC. The activity of LacZ knock-in reflecting endogenous expression of genes of interest in the whole body can be visualized by X-gal staining. This protocol is suitable for examining the developmental stage-specific expression of genes of interest spatially and temporally. For complete details on the use and execution of this protocol, please refer to Watanabe-Takano et al. (2021).

摘要

在这里，我们描述了一种使用 CUBIC 对组织透明胚胎和成年小鼠进行 X-gal 染色的优化方案。通过 X-gal 染色，可以观察到反映感兴趣基因在整个体内内源性表达的 LacZ 敲入活性。该方案适用于在空间和时间上检测感兴趣基因的发育阶段特异性表达。有关此方案使用和执行的完整详细信息，请参阅 Watanabe-Takano 等人（2021 年）。