School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules and National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai, 200240, P. R. China.
Department of Traumatology, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, P. R. China.
Small. 2022 Sep;18(36):e2107640. doi: 10.1002/smll.202107640. Epub 2022 Feb 4.
The cell microenvironment plays a crucial role in regulating cell behavior and fate in physiological and pathological processes. As the fundamental component of the cell microenvironment, extracellular matrix (ECM) typically possesses complex ordered structures and provides essential physical and chemical cues to the cells. Hydrogels have attracted much attention in recapitulating the ECM. Compared to natural and synthetic polymer hydrogels, DNA hydrogels have unique programmable capability, which endows the material precise structural customization and tunable properties. This review focuses on recent advances in programmable DNA hydrogels as artificial extracellular matrix, particularly the pure DNA hydrogels. It introduces the classification, design, and assembly of DNA hydrogels, and then summarizes the state-of-the-art achievements in cell encapsulation, cell culture, and tissue engineering with DNA hydrogels. Ultimately, the challenges and prospects for cellular applications of DNA hydrogels are delivered.
细胞微环境在生理和病理过程中对细胞行为和命运起着至关重要的调节作用。作为细胞微环境的基本组成部分，细胞外基质（ECM）通常具有复杂有序的结构，并为细胞提供必要的物理和化学线索。水凝胶在模拟 ECM 方面引起了广泛关注。与天然和合成聚合物水凝胶相比，DNA 水凝胶具有独特的可编程能力，赋予材料精确的结构定制和可调的性能。本综述重点介绍了可编程 DNA 水凝胶作为人工细胞外基质的最新进展，特别是纯 DNA 水凝胶。它介绍了 DNA 水凝胶的分类、设计和组装，然后总结了 DNA 水凝胶在细胞包封、细胞培养和组织工程方面的最新研究进展。最后，探讨了 DNA 水凝胶在细胞应用方面面临的挑战和前景。
