Role of prostacyclin on steroidogenesis by frog interrenal gland in vitro.

The role of prostacyclin (PGI2) on amphibian adrenal steroidogenesis was studied in vitro in perifused interrenal fragments from adult male frogs. Exogenous PGI2 (3 X 10(-8) M to 3 X 10(-5) M) and, in a lesser extent, 6-keto-PGF1 alpha increased both corticosterone and aldosterone production in a dose-related manner. Short pulses (20 min) of 0.88 microM PGI2 administered at 90 min intervals within the same experiment did not induce any desensitization phenomenon. A prolonged administration (6 h) of PGI2 gave rise to an important increase in steroid production followed by a decline of corticosteroidogenesis. Indomethacin (IDM, 5 microM) induced a marked reduction of the spontaneous secretion of corticosteroid which confirmed the involvement of endogenous PGs in the process of corticosteroid biosynthesis. The IDM-induced blockade of corticosterone and aldosterone secretion was totally reversed by administration of exogenous PGI2 in our model. Angiotensin II (AII) induced a massive release of 6-keto-PGF1 alpha, the stable metabolite of PGI2. The increase of 6-keto-PGF1 alpha preceded the stimulation of corticosterone and aldosterone secretions. In contrast, the administration of ACTH did not modify the release of 6-keto-PGF1 alpha. These results indicate that PGI2 might be an important mediator of adrenal steroidogenesis in frog. They confirm that the corticosteroidogenic actions of ACTH and AII are mediated by different mechanisms.