胫后神经刺激通过瞬时受体电位/P2X 信号通路改善脊髓损伤大鼠的神经性膀胱。
Posterior tibial nerve stimulation improves neurogenic bladder in rats with spinal cord injury through transient receptor potential/P2X signaling pathway.
机构信息
Department of Rehabilitation Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei, China.
Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China.
出版信息
Neurourol Urodyn. 2022 Mar;41(3):756-764. doi: 10.1002/nau.24885. Epub 2022 Feb 7.
BACKGROUND
To study the influences of posterior tibial nerve stimulation (PTNS) on neurogenic bladder and the expression of transient receptor potential (TRP) channels and P2X receptors in rats with spinal cord injury (SCI) and explore the possible mechanism.
METHODS
SCI model was established by modified Allen's method and PTNS was performed. Urodynamic indexes and Haematoxylin and Eosine staining of bladder tissue were used to evaluate the therapeutic effect. The expression of TRP channels and P2X receptors in the bladder and dorsal root ganglia (DRG) was detected by real-time PCR and Western blot.
RESULTS
The low compliance of bladder in treatment group was significantly improved compared with SCI group, and the infiltration of inflammatory cells in bladder tissue was significantly reduced. At the same time, the expression of TRP and P2X in bladder and DRG was partially restored after the treatment of PTNS.
CONCLUSIONS
PTNS is an effective therapy for SCI-induced neurogenic bladder via the TRP/P2X signaling pathway.
背景
研究胫骨后神经刺激（PTNS）对脊髓损伤（SCI）大鼠神经性膀胱的影响及瞬时受体电位（TRP）通道和 P2X 受体的表达，探讨其可能的机制。
方法
采用改良 Allen 法建立 SCI 模型，并进行 PTNS 治疗。采用尿动力学指标和膀胱组织苏木精-伊红（HE）染色评价治疗效果。实时 PCR 和 Western blot 检测膀胱和背根神经节（DRG）中 TRP 通道和 P2X 受体的表达。
结果
与 SCI 组相比，治疗组的膀胱顺应性明显改善，膀胱组织中炎性细胞浸润明显减少。同时，PTNS 治疗后，膀胱和 DRG 中 TRP 和 P2X 的表达部分恢复。
结论
TRP/P2X 信号通路可能参与了 PTNS 治疗 SCI 诱导的神经性膀胱的过程，PTNS 是一种有效的治疗方法。
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