芍药花叶卷叶相关病毒全基因组序列，I 亚组中的一种新的马铃薯 Y 病毒属病毒。
Complete genome sequence of peony leafroll-associated virus, a novel ampelovirus in subgroup I.
机构信息
College of Bioscience and Resource Environment, Beijing University of Agriculture, Beijing, 102206, China.
Key Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs, Beijing University of Agriculture, Beijing, 102206, China.
出版信息
Arch Virol. 2022 Mar;167(3):995-998. doi: 10.1007/s00705-022-05389-8. Epub 2022 Feb 8.
The complete genome sequence of peony leafroll-associated virus (PLRaV) was determined by deep sequencing of ribosomal-RNA-depleted total RNA extracted from a peony plant exhibiting leafroll symptoms. Further PCR and RACE analysis showed that the PLRaV genome consists of 15,406 nucleotides and contains 10 putative open reading frames, with an organization typical of members of the genus Ampelovirus, family Closteroviridae. Amino acid sequence comparisons showed that the viral heat shock protein 70 homolog (HSP70h) shared the highest sequence identity (41.7%) with the corresponding region of grapevine leafroll-associated virus 1, and the coat protein (CP) and RNA-dependent RNA polymerase (RdRp) shared the highest sequence identity (32.1% and 52.3%, respectively) with grapevine leafroll-associated virus 13. Phylogenetic analysis of the HSP70h, CP, and RdRp aa sequences showed that PLRaV clustered with members of subgroup I of the genus Ampelovirus.
牡丹叶卷相关病毒（PLRaV）的全基因组序列通过对表现叶卷症状的牡丹植株中核糖体 RNA 耗尽的总 RNA 进行深度测序来确定。进一步的 PCR 和 RACE 分析表明，PLRaV 基因组由 15406 个核苷酸组成，包含 10 个推定的开放阅读框，其组织与 Closteroviridae 科 Ampelovirus 属的成员典型。氨基酸序列比较表明，病毒热休克蛋白 70 同源物（HSP70h）与葡萄叶卷相关病毒 1 的相应区域具有最高的序列同一性（41.7%），而外壳蛋白（CP）和 RNA 依赖性 RNA 聚合酶（RdRp）与葡萄叶卷相关病毒 13 的相应区域具有最高的序列同一性（分别为 32.1%和 52.3%）。HSP70h、CP 和 RdRp aa 序列的系统发育分析表明，PLRaV 与 Ampelovirus 属 I 亚组的成员聚类。
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