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来自……的……在……中的过表达影响叶片形态的发育。 (由于原文部分关键信息缺失,翻译可能不太准确完整)

Overexpression of from in affects the development of leaf morphology.

作者信息

Zhou Rui, Fan Menglong, Zhao Mei, Jiang Xinqiang, Liu Qinghua

机构信息

College of Landscape Architecture and Forestry, Qingdao Agricultural University, Qingdao, Shandong, China.

State Key Laboratory of Tree Genetics and Breeding, Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, Zhejiang, China.

出版信息

Plant Signal Behav. 2022 Dec 31;17(1):2031783. doi: 10.1080/15592324.2022.2031783. Epub 2022 Feb 10.

Abstract

Leaves are the main vegetative organs of the aboveground part of plants and play an important role in plant morphogenesis. () plays a crucial role in regulating leaf cell fate and maintaining leaf development. In this study, we analyzed from and illustrated its function in transgenic plants. Tissue-specific expression analysis indicated that was highly expressed in stems, young flower buds, and shoot apical meristems (SAMs). Ectopic overexpression of in suggested that transformants with mild phenotypes were characterized by foliar wrinkles and mildly curled leaves; transformants with intermediate phenotypes showed severely crimped blades and narrow leaf angles, and the most severe phenotypes lacked normal SAMs and leaves. Moreover, the expression levels of genes involved in the regulation of KNOX in transgenic plants were detected, including and (), and the expression of other genes were down-regulated except . This study contributes to our understanding of the function.

摘要

叶片是植物地上部分的主要营养器官,在植物形态发生中起重要作用。()在调节叶细胞命运和维持叶片发育方面起着关键作用。在本研究中,我们从()分析了()并阐述了其在转基因植物中的功能。组织特异性表达分析表明,()在茎、幼花芽和茎尖分生组织(SAMs)中高度表达。()在()中的异位过表达表明,具有轻度表型的转化体的特征是叶面有皱纹和叶片轻度卷曲;具有中间表型的转化体表现出叶片严重卷曲和叶角变窄,最严重的表型则缺乏正常的SAMs和叶片。此外,检测了转基因植物中参与KNOX调控的基因的表达水平,包括()和(),除了()外,其他基因的表达均下调。本研究有助于我们对()功能的理解。

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