父母和新生儿卡介苗接种与新生儿辅助性 T 细胞 17 细胞扩增之间的关联。

The association between parental and neonatal BCG vaccination and neonatal T helper 17 cell expansion.

机构信息

Bandim Health Project, INDEPTH Network, Bissau, Guinea-Bissau; Bandim Health Project, OPEN, Institute of Clinical Research, Uni. Southern Denmark and Odense University Hospital, Odense, Denmark.

Department of Neonatology, Birmingham Women's and Children's NHS Foundation Trust, Birmingham, UK; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK; Liggins Institute, University of Auckland, Auckland, New Zealand.

出版信息

Vaccine. 2022 Mar 15;40(12):1799-1804. doi: 10.1016/j.vaccine.2022.02.007. Epub 2022 Feb 10.

DOI:10.1016/j.vaccine.2022.02.007

PMID:35151505

Abstract

BACKGROUND

Bacille Calmette-Guérin (BCG) vaccination reduces the severity of neonatal infections; this effect appears enhanced if the mother has received BCG. We performed immunophenotyping of the T-cell subset and characterized T-cell proliferation responses to assess possible immune response pathways.

METHODS

Healthy BCG-vaccinated (n = 8) and unvaccinated (n = 9) neonates born by elective caesarean section were sampled 3 weeks after birth. We compared a wide panel of intracellular cytokine and cell surface expression markers as well as proliferation response in T-cells between BCG-vaccinated and unvaccinated neonates, stratified by parental BCG status.

RESULTS

For all BCG-vaccinated neonates and 3 of 9 unvaccinated neonates that served as controls, both parents had a BCG scar. Th17 (CD4 + IL-17+) prevalence as percentage of total CD4 + T-cells was expanded 4-fold in BCG-vaccinated compared to unvaccinated, being 11.6% [3.6-19.6%] vs 2.8% [1.0-6.6%]. Th17 counts for 3 unvaccinated neonates born to BCG-vaccinated parents was comparable to vaccinated neonates, and higher than remaining controls, parental BCG = 8.5% [4.4-8.9%] vs 1.8% [0.8-3.3%] for no parental BCG (median [interquartile range] for all data).

CONCLUSION

Among neonates born to BCG-vaccinated parents, the prevalence of Th17 cells, important in the response against bacterial infections, was substantially elevated. The interaction between neonatal and parental BCG for Th17 responses and the importance remains to be further investigated.

摘要

背景

卡介苗（BCG）接种可减轻新生儿感染的严重程度；如果母亲接受过 BCG 接种，这种效果似乎会增强。我们进行了 T 细胞亚群免疫表型分析，并对 T 细胞增殖反应进行了特征描述，以评估可能的免疫反应途径。

方法

通过择期剖宫产分娩的健康 BCG 接种（n=8）和未接种（n=9）新生儿在出生后 3 周时进行采样。我们比较了广泛的细胞内细胞因子和细胞表面表达标志物面板，以及 T 细胞的增殖反应，以评估 BCG 接种和未接种新生儿之间的差异，并按父母的 BCG 状态进行分层。

结果

对于所有 BCG 接种的新生儿和 9 名未接种的对照新生儿中的 3 名，其父母均有 BCG 疤痕。与未接种组相比，BCG 接种组 Th17（CD4+IL-17+）的百分比增加了 4 倍，为 11.6%[3.6-19.6%]，而未接种组为 2.8%[1.0-6.6%]。3 名未接种的新生儿出生于 BCG 接种的父母，其 Th17 计数与接种组相似，高于其余对照组，父母 BCG=8.5%[4.4-8.9%]，而无父母 BCG 为 1.8%[0.8-3.3%]（所有数据的中位数[四分位数范围]）。