菜籽粕中硫代葡萄糖苷的测定及其被菜籽芽中黑芥子酶的降解
Determination of glucosinolates in rapeseed meal and their degradation by myrosinase from rapeseed sprouts.
作者信息
Xie Chong, Li Wanmei, Gao Rongguang, Yan Lihua, Wang Pei, Gu Zhenxin, Yang Runqiang
机构信息
College of Food Science and Technology, Whole Grain Food Engineering Research Center, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China.
College of Food Science and Technology, Whole Grain Food Engineering Research Center, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People's Republic of China.
出版信息
Food Chem. 2022 Jul 15;382:132316. doi: 10.1016/j.foodchem.2022.132316. Epub 2022 Feb 4.
The utilization of rapeseed meal in food is limited due to its abundant glucosinolates (GLs). In this study, an LC-MS/MS method for GLs determination in rapeseed meal was developed. Then, the degradation of GLs using rapeseed sprouts derived myrosinase (MYR) was investigated. Results showed that 11 kinds of GLs were identified in rapeseed meal. The LC-MS/MS method had a high linearity (R greater than 0.9999), repeatability (RSD < 5%) and recovery rate (92%-102%). The optimum condition for hydrolyzing GLs in rapeseed meal was reacting for 4 h with the addition of 2236.35 U/g MYR, 9.63 μg/g ascorbic acid and 26.68 μg/g EDTA. Under this condition, more than 80% of GLs were degraded and the yields of isothiocyanates and oxazolidinone-2-thione were 859.30 μg/g and 685.59 μg/g, respectively. To conclude, this study reported a reliable method for GLs determination and an effective way to degrade GLs in rapeseed meal.
由于菜籽粕中含有大量硫代葡萄糖苷（GLs），其在食品中的应用受到限制。本研究建立了一种用于测定菜籽粕中硫代葡萄糖苷的液相色谱-串联质谱（LC-MS/MS）方法。然后，研究了利用菜籽芽来源的黑芥子酶（MYR）对硫代葡萄糖苷的降解情况。结果表明，在菜籽粕中鉴定出11种硫代葡萄糖苷。该LC-MS/MS方法具有高线性（R大于0.9999）、重复性（RSD<5%）和回收率（92%-102%）。水解菜籽粕中硫代葡萄糖苷的最佳条件是加入2236.35 U/g黑芥子酶、9.63μg/g抗坏血酸和26.68μg/g乙二胺四乙酸（EDTA）反应4小时。在此条件下，超过80%的硫代葡萄糖苷被降解，异硫氰酸盐和恶唑烷-2-硫酮的产量分别为859.30μg/g和685.59μg/g。总之，本研究报道了一种可靠的硫代葡萄糖苷测定方法以及一种有效降解菜籽粕中硫代葡萄糖苷的方法。
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