Kivelä T
Acta Ophthalmol (Copenh). 1986 Feb;64(1):19-25. doi: 10.1111/j.1755-3768.1986.tb06866.x.
Forty-six retinoblastoma specimens were studied for the presence of neuron-specific enolase (NSE) in the tumour cells and in the remaining morphologically normal retina. The laboratory method was a sensitive immunohistochemical peroxidase staining procedure, and normal ocular nerves were used as positive controls. Neuron-specific enolase was found in perikarya and cell processes in all layers of morphologically normal appearing neuroretina, but not in Müller's cells. All but two of the retinoblastomata studied contained positively staining tumour cells, whereas stromal cells remained constantly negative. Many Flexner-Wintersteiner rosettes stained positively as well. The proportion of retinoblastoma cells expressing neuron-specific enolase seemed to be greater in small tumours, in bilateral cases, and in tumours that contained rosettes. The presence of neuron-specific enolase in tumour cells seems to favour a neuronal or neuroectodermal origin for retinoblastoma, but it should be noted that this marker has recently been found in several non-neuronal and non-neuroectodermal malignant neoplasms.
对46份视网膜母细胞瘤标本进行研究，以检测肿瘤细胞及剩余形态学正常的视网膜中神经元特异性烯醇化酶（NSE）的存在情况。实验方法为敏感的免疫组织化学过氧化物酶染色程序，正常眼神经用作阳性对照。在形态学正常的神经视网膜各层的核周体和细胞突起中发现了神经元特异性烯醇化酶，但在米勒细胞中未发现。所研究的视网膜母细胞瘤中，除两份外，其余均含有呈阳性染色的肿瘤细胞，而基质细胞始终呈阴性。许多Flexner-Wintersteiner菊形团也呈阳性染色。在小肿瘤、双侧病例以及含有菊形团的肿瘤中，表达神经元特异性烯醇化酶的视网膜母细胞瘤细胞比例似乎更高。肿瘤细胞中神经元特异性烯醇化酶的存在似乎支持视网膜母细胞瘤起源于神经元或神经外胚层，但应注意的是，最近在几种非神经元和非神经外胚层恶性肿瘤中也发现了这种标志物。
