Boivin P, Bourdillon C
Biochem Biophys Res Commun. 1986 Mar 28;135(3):928-33. doi: 10.1016/0006-291x(86)91017-x.
The linear sweep voltammetric method is used as a new approach for kinetic determination with enzymes accepting reversible redox couples as cosubstrate. A monolayer of hydrogenase molecules is grafted onto a glassy carbon electrode which is both the support of the enzyme and the detector of the activity. Reduced viologen concentration in the enzyme microenvironment is controlled by the electrode potential. The catalytic current produced by the enzyme allows an easy kinetic constant determination without the classical constraints found in hydrogenase kinetic measurements.
线性扫描伏安法被用作一种新的动力学测定方法，用于以接受可逆氧化还原对作为共底物的酶进行测定。将单层氢化酶分子接枝到玻碳电极上，该电极既是酶的载体又是活性检测器。酶微环境中还原态紫精的浓度由电极电位控制。酶产生的催化电流使得能够轻松测定动力学常数，而无需氢化酶动力学测量中存在的传统限制。
