Vieira Diego Henrique Mirandola Dias, Narciso Rodrigo Bravin, da Silva Reinaldo José
São Paulo State University (UNESP), Institute of Biosciences, Division of Parasitology, 18618-689 Botucatu, SP, Brazil.
São Paulo State University (UNESP), Institute of Biosciences, Division of Parasitology, 18618-689 Botucatu, SP, Brazil.
Parasitol Int. 2022 Jun;88:102560. doi: 10.1016/j.parint.2022.102560. Epub 2022 Feb 12.
We describe Myxobolus cataractae n. sp., found parasitizing Imparfinis mirini from Cachoeirinha stream, a tributary of Pardo River, Paraná River basin, municipality of Botucatu, São Paulo State, Brazil. We based the description on myxospore morphology, histology, and small subunit ribosomal DNA partial sequences. Whitish and elongated mass of myxospores were found in gill filaments, occupying an intrafilamental position. The myxospores were slightly ellipsoidal and the morphometric measurements revealed a myxospore body length of 7.8 ± 0.4 μm; myxospore width of 5.9 ± 0.4 μm; and thickness of 3.9 ± 0.3 μm. The polar capsules are equal in size and measured 3.5 ± 0.2 μm in length and 1.7 ± 0.2 μm in width. The polar tubules had 6-7 coils. Myxobolus cataractae n. sp. is classified as the intrafilamental type and chondreal subtype by the histological analysis of gills. Phylogenetic analysis showed a well-supported subclade formed by species that parasitize gills of Siluriformes, with M. imparfinis as a sister species of Myxobolus cataractae n. sp. Using molecular and morphological characterization, this cryptic species was identified as a new species of the genus Myxobolus.
我们描述了新种卡塔拉克特碘泡虫（Myxobolus cataractae n. sp.），它寄生于巴西圣保罗州博图卡图市巴拉那河流域帕尔多河支流卡舒埃里尼亚溪流中的米氏无鳍鲶（Imparfinis mirini）。我们基于粘孢子形态、组织学以及小亚基核糖体DNA部分序列进行描述。在鳃丝中发现了白色细长的粘孢子团块，占据丝内位置。粘孢子略呈椭圆形，形态测量显示粘孢子体长7.8±0.4μm；粘孢子宽5.9±0.4μm；厚度3.9±0.3μm。极囊大小相等，长3.5±0.2μm，宽1.7±0.2μm。极丝有6 - 7圈。通过鳃的组织学分析，卡塔拉克特碘泡虫新种被归类为丝内型和软骨亚型。系统发育分析显示，由寄生于鲇形目鳃的物种形成了一个得到充分支持的亚分支，米氏无鳍鲶是卡塔拉克特碘泡虫新种的姐妹种。通过分子和形态特征鉴定，这个隐存种被确定为碘泡虫属的一个新物种。
