Overexpression of ZmPCK2, a phosphoenolpyruvate carboxykinase gene from maize confers enhanced tolerance to water deficit stress in rice.

Water deficit is one of the major abiotic stresses that limit plant growth and global crop yields. Phosphoenolpyruvate carboxykinase (PCK) plays important roles in regulating plant growth and development, but its role in water-deficit stress remains elusive. In this study, we found that overexpression of ZmPCK2 significantly enhanced the water-deficit tolerance of transgenic rice. The expression level of ZmPCK2 was strongly induced by PEG and ABA treatments. Overexpression of ZmPCK2 in rice increased stomatal closure and water saving by regulating malate metabolism under water-deficit conditions. Moreover, the expression of ZmPCK2 in rice up-regulated ABA biosynthesis and responsive genes under water-deficit stress, and ZmPCK2 transgenic rice showed hypersensitive to exogenous ABA at germination stage, suggesting that ZmPCK2 may be involved in ABA signalling pathway. Under water-deficit stress, the ZmPCK2 transgenic rice showed higher antioxidant enzyme activities and lower accumulation of reactive oxygen species (ROS) compared with non-transgenic (NT) plants, resulting in less oxidative damage. Taken together, we suggest that ZmPCK2 plays multiple roles in response to water-deficit stress by enhancing ABA signalling pathway, regulating malate metabolism, promoting stomatal closure and further activating the ROS-scavenging system.