Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, University of South Carolina School of Medicine/Prisma Health, Greenville, South Carolina.
Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, La Jolla, San Diego, California.
F S Sci. 2021 Aug;2(3):230-236. doi: 10.1016/j.xfss.2021.06.004. Epub 2021 Jun 30.
To study the effects of ibuprofen on androgen production, gene expression, and cell viability in rat theca-interstitial cells exposed to the proinflammatory stimuli interleukin-1 (IL-1) and lipopolysaccharide (LPS).
Animal study.
University-based research laboratory.
PATIENTS/ANIMALS: Theca-interstitial cells were isolated from 30 day old female Sprague Dawley rats.
Theca cells were cultured with pro-inflammatory media containing IL-1 and LPS and compared with cells cultured in control media.
Androstenedione quantification was performed on conditioned cell culture medium using liquid chromatography-mass spectrometry. Theca cell viability was assessed using PrestoBlue cell viability assay. The gene expression of , , and was analyzed using quantitative polymerase chain reaction.
Both proinflammatory stimuli IL-1 and LPS increased androstenedione concentration in cell culture medium, and these effects were mitigated with ibuprofen. Both inflammatory agents in addition increased the expression of key genes involved in androgen synthesis: , , and ; the addition of ibuprofen to the culture medium inhibited these effects. Theca cell viability increased with IL-1 and LPS. Ibuprofen inhibited the IL-1-mediated increase in cell viability but did not reverse the effects of LPS.
In conclusion, our findings support the hypothesis that many of the alterations induced by inflammatory stimuli in theca-interstitial cells are abrogated by the addition of ibuprofen.
研究布洛芬对白细胞介素-1（IL-1）和脂多糖（LPS）刺激的大鼠卵巢间质细胞雄激素产生、基因表达和细胞活力的影响。
动物研究。
大学研究实验室。
患者/动物：从 30 天大的雌性 Sprague Dawley 大鼠中分离卵巢间质细胞。
用含有促炎介质 IL-1 和 LPS 的培养基培养间质细胞，并与培养在对照培养基中的细胞进行比较。
使用液相色谱-质谱法对条件培养基中的雄烯二酮进行定量。使用 PrestoBlue 细胞活力测定法评估卵巢间质细胞活力。使用定量聚合酶链反应分析 、 和 的基因表达。
两种促炎刺激物 IL-1 和 LPS 均增加了细胞培养基中的雄烯二酮浓度，而布洛芬可减轻这些作用。两种炎症因子均增加了雄激素合成关键基因的表达： 、 和 ；向培养基中添加布洛芬可抑制这些作用。IL-1 和 LPS 增加了卵巢间质细胞的活力。布洛芬抑制了 IL-1 介导的细胞活力增加，但未能逆转 LPS 的作用。
总之，我们的研究结果支持这样一种假设，即许多由炎症刺激物引起的卵巢间质细胞的改变都可以通过添加布洛芬来消除。
