United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima 890-0065, Japan.
Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
Biosensors (Basel). 2022 Jan 18;12(2):49. doi: 10.3390/bios12020049.
Early and quick detection of pathogens are crucial for managing the spread of infections in the biomedical, biosafety, food, and agricultural fields. While molecular diagnostics can offer the specificity and reliability in acute infectious diseases, detection of pathogens is often slowed down by the current benchtop molecular diagnoses, which are time consuming, labor intensive, and lack the mobility for application at the point-of-need. In this work, we developed a complete on-farm use detection protocol for the plant-devastating anthracnose agent: . Our methods combined a simplified DNA extraction on paper that is compatible with loop-mediated isothermal amplification (LAMP), coupled with paper-based immunoassay lateral flow sensing. Our results offer simple, quick, easy, and a minimally instrumented toolkit for detection. This scalable and adaptable platform is a valuable alternative to traditional sensing systems towards on-the-go pathogen detection in food and agriculture, biomedical, and other fields.
早期和快速检测病原体对于控制生物医学、生物安全、食品和农业领域的感染传播至关重要。虽然分子诊断可以在急性传染病中提供特异性和可靠性，但目前的台式分子诊断往往会因耗时、劳动强度大以及缺乏在需要时进行现场应用的机动性而导致病原体检测变得缓慢。在这项工作中，我们开发了一种针对植物破坏性炭疽病病原体的完整农场使用检测方案：. 我们的方法结合了适用于环介导等温扩增 (LAMP) 的纸张上简化的 DNA 提取，以及基于纸张的免疫测定侧向流动传感。我们的结果提供了一种简单、快速、易用且仪器化程度最低的工具包，用于 检测。这种可扩展和可适应的平台是传统传感系统的一个有价值的替代品，可用于食品和农业、生物医学和其他领域的现场病原体检测。
