L改善气道高反应性并抑制过敏性哮喘小鼠模型中的炎症。
L. improves airway hyperresponsiveness and suppresses inflammation in a murine model of allergic asthma.
作者信息
Frota Clara do Prado Lopes, Ribeiro Alessa Castro, Filho Marco Túlio Alvarenga Calil, Morel Lucas Junqueira de Freitas, Ramalho Leandra Naira Zambelli, Amaral Jéssica Cristina, Contini Silvia Helena Taleb, da Fonseca Vanessa Maciel Braulio, Borges Marcos de Carvalho, Pereira Ana Maria Soares, Carmona Fabio
机构信息
Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, Brazil.
Department of Plant Biotechnology, University of Ribeirao Preto, Ribeirao Preto, Brazil.
出版信息
Allergol Immunopathol (Madr). 2022 Mar 1;50(2):115-123. doi: 10.15586/aei.v50i2.577. eCollection 2022.
OBJECTIVE
To evaluate the effect of a hydroethanolic extract of L. ("bitter melon", Cucurbitaceae) leaves (MCHA) on ovalbumin (OVA)-induced asthma model. Balb/c mice were sensitized twice and challenged for 4 alternate days with OVA and then treated with MCHA (500 mg/kg) for 7 consecutive days.
METHODS
Control groups received treatment with normal saline or dexamethasone (2 mg/kg) on the same day. We assessed bronchial hyperresponsiveness and inflammation and mucus production in bronchoalveolar lavage (BAL), lung homogenates, and lung tissue.
RESULTS
MCHA significantly improved airway hyperresponsiveness near baseline levels. MCHA administration significantly improved airway and lung inflammation, demonstrated by decreased total and inflammatory cells in BAL, lower levels of IL-5 and IL-13 in lung homogenate, and fewer inflammatory cells in lung tissue. Additionally, MCHA significantly diminished goblet cells in lung tissue.
CONCLUSIONS
Administration of a hydroethanolic extract of leaves was effective in treating OVA-induced asthma in an animal model.
目的
评估葫芦科植物“苦瓜”（L.）叶片的水乙醇提取物（MCHA）对卵清蛋白（OVA）诱导的哮喘模型的影响。将Balb/c小鼠致敏两次，并用OVA连续4天隔天进行激发，然后连续7天用MCHA（500mg/kg）治疗。
方法
对照组在同一天接受生理盐水或地塞米松（2mg/kg）治疗。我们评估了支气管高反应性以及支气管肺泡灌洗（BAL）、肺匀浆和肺组织中的炎症和黏液产生情况。
结果
MCHA显著改善气道高反应性，使其接近基线水平。给予MCHA显著改善气道和肺部炎症，表现为BAL中总细胞和炎性细胞减少、肺匀浆中IL-5和IL-13水平降低以及肺组织中炎性细胞减少。此外，MCHA显著减少肺组织中的杯状细胞。
结论
给予叶片的水乙醇提取物对动物模型中OVA诱导的哮喘具有治疗效果。
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