College of Pharmacy, Henan University of Chinese Medicine, 156 Esat Jin-shui Rd, Zhengzhou 450046, PR China; Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Henan University of Chinese Medicine, 156 Esat Jin-shui Rd, Zhengzhou 450046, PR China; Henan Key Laboratory of Chinese Medicine Resources and Chemistry, 156 Esat Jin-shui Rd, Zhengzhou 450046, PR China.
College of Pharmacy, Henan University of Chinese Medicine, 156 Esat Jin-shui Rd, Zhengzhou 450046, PR China.
Int Immunopharmacol. 2022 Jun;107:108673. doi: 10.1016/j.intimp.2022.108673. Epub 2022 Mar 5.
Lindera reflexa Hemsl. (LR) has been used for the treatment of gastrointestinal disorders. The present study was carried out to investigate the gastroprotective effect of an active ingredients group of Lindera reflexa Hemsl. (LRG) on ethanol-induced gastric ulcer in rats and its possible mechanisms. The ulcer area was measured, and samples of gastric tissue were taken for histochemical, pathological, and biochemical analyses. Pretreatment with LRG protected the gastric mucosa as seen by reduction the GUI and gastric juice volume, regulated gastric acid secretion. LRG counteracted the ethanol-induced oxidative stress by increasing the levels of depleted SOD and CAT as well as significantly attenuating the lipid peroxidation by reducing the levels of MDA and MPO. LRG also reduced release of inflammatory mediator TNF-α, increased the content of PGE and inhibited MTL secretion. Immunofluorescence and Western blot analyses confirmed that the co-localization of TLR-2 and MyD88 protein in the gastric mucosa of LRG-treated rats was significantly lower than that of rats with gastric ulcers. Furthermore, LRG also modulated the expression of Ki-67 antigens. LRG markedly increased the expression of phosphorylated form of extracellular signal-regulated kinaseVEGFR2, ERK1/2, AKT and p38, thereby protecting the gastric mucosa. These findings indicated that the gastroprotective effect of LRG is attributable to its antioxidant, anti-inflammatory, and antisecretory properties. In addition, LRG can ameliorate ethanol-induced gastric ulcers in rats by regulating the VEGFR2/ERK and TLR-2/MyD88 signaling pathways.
山胡椒（LR）已被用于治疗胃肠道疾病。本研究旨在探讨山胡椒有效成分组（LRG）对乙醇诱导的大鼠胃溃疡的胃保护作用及其可能机制。测量溃疡面积，并取胃组织样本进行组织化学、病理和生化分析。LRG 通过减少 GUI 和胃液量、调节胃酸分泌来保护胃黏膜。LRG 通过增加耗竭 SOD 和 CAT 的水平以及通过降低 MDA 和 MPO 的水平来显著减轻脂质过氧化来对抗乙醇诱导的氧化应激。LRG 还降低了促炎介质 TNF-α的释放，增加了 PGE 的含量并抑制了 MTL 的分泌。免疫荧光和 Western blot 分析证实，与胃溃疡大鼠相比，LRG 处理大鼠胃黏膜中 TLR-2 和 MyD88 蛋白的共定位明显降低。此外，LRG 还调节了 Ki-67 抗原的表达。LRG 显著增加了磷酸化形式的细胞外信号调节激酶 VEGFR2、ERK1/2、AKT 和 p38 的表达，从而保护胃黏膜。这些发现表明，LRG 的胃保护作用归因于其抗氧化、抗炎和抗分泌特性。此外，LRG 可以通过调节 VEGFR2/ERK 和 TLR-2/MyD88 信号通路来改善乙醇诱导的大鼠胃溃疡。
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