[耐辐射大肠杆菌Gamr444菌株中的氧效应]

[Oxygen effect in the radioresistant Escherichia coli Gamr444 strain].

作者信息

Korystov Iu N, Veksler F B

出版信息

Radiobiologiia. 1986 May-Jun;26(3):372-4.

Abstract

In experiments on E. coli Gamr 444 the oxygen effect has been studied. Cells were exposed to gamma-rays with constant bubbling of oxygen or nitrogen through the suspension or without bubbling. In the latter case the dose-effect curve was distorted due to radiochemical absorption of oxygen. The dose curve parameters were determined in the anoxic and oxygenating conditions, they are: lin n(N2) = 3.6; D0(N2) = 371 Gy; In n(O2) = 3.6; D0(O2) = 112 Gy. The oxygen effect for E. coli Gamr 444 was 3.3 as determined by D0. In studying the radiosensitivity and its modification in radioresistant strains one should eliminate the influence of radiochemical absorption of oxygen by aeration of the medium during exposure.

摘要

在对大肠杆菌Gamr 444进行的实验中，研究了氧效应。细胞暴露于γ射线，通过悬浮液持续鼓入氧气或氮气，或不鼓入气体。在后一种情况下，由于氧气的放射化学吸收，剂量效应曲线发生了畸变。在缺氧和充氧条件下测定了剂量曲线参数，它们是：ln n(N₂)=3.6；D₀(N₂)=371 Gy；ln n(O₂)=3.6；D₀(O₂)=112 Gy。由D₀确定，大肠杆菌Gamr 444的氧效应为3.3。在研究抗辐射菌株的放射敏感性及其修饰时，应在暴露期间通过对培养基通气消除氧气放射化学吸收的影响。