在体内生成正电子发射断层扫描（PET）的铈（Ce）和钕（Nd）标记在一种内化单克隆抗体探针上。
PET in vivo generators Ce and Nd on an internalizing monoclonal antibody probe.
作者信息
Severin G W, Fonslet J, Kristensen L K, Nielsen C H, Jensen A I, Kjær A, Mazar A P, Johnston K, Köster U
机构信息
The Hevesy Laboratory, DTU Health Technology, Technical University of Denmark (DTU), Frederiksborgvej 399, 4000, Roskilde, Denmark.
Department of Chemistry, Michigan State University, East Lansing, MI, USA.
出版信息
Sci Rep. 2022 Mar 9;12(1):3863. doi: 10.1038/s41598-022-07147-x.
The in vivo-generator radionuclides Nd (t = 3.4 d) and Ce (t = 3.2 d) were used to trace a urokinase-type plasminogen activator (uPA)-targeting mouse monoclonal antibody, ATN-291, in U87 MG xenograft tumor-bearing mice. ATN-291 is known to internalize on the uPA/uPA-receptor pair, making it an appropriate targeting vector for investigating the fate of in vivo generator daughters on internalizing probes. Ante-mortem and post-mortem PET imaging at 120 h post-injection gave no indication of redistribution of the positron emitting daughter nuclides La and Pr from tumor tissue (p > 0.5). The lack of redistribution indicates that the parent radionuclides Ce and Nd could be considered as long-lived PET-diagnostic matches to therapeutic radionuclides like Lu, Tb and Ac when internalizing bioconjugates are employed.
使用体内发生器放射性核素钕（t = 3.4天）和铈（t = 3.2天）在携带U87 MG异种移植肿瘤的小鼠中追踪一种靶向尿激酶型纤溶酶原激活剂（uPA）的小鼠单克隆抗体ATN-291。已知ATN-291在uPA/uPA受体对上内化，这使其成为研究体内发生器子代在内化探针上的命运的合适靶向载体。注射后120小时的生前和死后PET成像未显示肿瘤组织中发射正电子的子代核素镧和镨的重新分布（p > 0.5）。缺乏重新分布表明，当使用内化生物共轭物时，母体放射性核素铈和钕可被视为与镥、铽和锕等治疗性放射性核素的长寿命PET诊断匹配物。
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