National Centre for Tissue and Cell Banking, Chalubinskiego 5, 02-004, Warsaw, Poland.
Department of Transplantology and Central Tissue Bank, Medical University of Warsaw, Chalubinskiego 5, 02-004, Warsaw, Poland.
Cell Tissue Bank. 2022 Dec;23(4):833-844. doi: 10.1007/s10561-022-09999-z. Epub 2022 Mar 10.
Ionizing radiation sterilization of non-defatted bone grafts has been found to deteriorate their quality and biocompatibility due to induction of lipid peroxidation products toxic for osteoblast-like cells. Therefore, the purpose of our study was to evaluate the effect of two types of ionizing radiation-gamma rays (G) or accelerated electron beam (EB) applied with two doses at different temperature conditions on hydrocarbons production, resulting from decomposition of palmitic and oleic acids-most abundant fatty acids in medullary lipids. Bone marrow samples isolated from femoral shafts of 6 male donors (aged 46-67 years) were irradiated with G or EB with doses of 25 or 35 kGy at different temperature conditions (ambient or deep freezing temperature). Fresh-frozen, non-irradiated samples served as control. Marrow lipids were extracted with n-hexane (Soxhlet's method), hydrocarbons fraction isolated on Florisil column chromatography, separated by gas chromatography and detected by mass spectrometry. Irradiation of bone marrow with sterilization doses of ionizing radiation (G and EB) was found to induce lipid radiolysis as measured by resulting hydrocarbons production. The effect was dose-dependent, whereas no marked influence of radiation type was observed. In contrast, irradiation temperature had a profound effect on lipids decomposition which was partially prevented while irradiation was performed in deep frozen state. Defatting of bone grafts prior to ionizing radiation sterilization seems essential for their biocompatibility, whereas irradiation in a deep-frozen state might compromise the effectiveness of sterilization and needs further studies.
非脱脂骨移植物的电离辐射灭菌会导致其质量和生物相容性恶化，这是由于诱导的脂质过氧化产物对成骨样细胞有毒。因此，我们的研究目的是评估两种类型的电离辐射-γ射线（G）或加速电子束（EB）在不同温度条件下应用两种剂量对烃类产生的影响，这些烃类是由骨髓脂质中最丰富的脂肪酸棕榈酸和油酸分解产生的。从 6 名男性供体（年龄 46-67 岁）的股骨中分离出骨髓样本，用 G 或 EB 以 25 或 35 kGy 的剂量在不同温度条件（环境或深冷冻温度）下进行照射。新鲜冷冻、未照射的样本作为对照。骨髓脂质用正己烷（索氏提取法）提取，烃类馏分用 Florisil 柱色谱分离，用气相色谱法分离，并通过质谱法检测。研究发现，骨髓的电离辐射（G 和 EB）灭菌剂量会导致脂质辐射分解，从而产生烃类产物。这种影响是剂量依赖性的，而辐射类型没有明显的影响。相比之下，辐照温度对脂质分解有深远的影响，而在深冷冻状态下进行辐照时，这种影响部分得到了预防。在进行电离辐射灭菌之前对骨移植物进行脱脂似乎对其生物相容性至关重要，而在深冷冻状态下进行辐照可能会影响灭菌的效果，需要进一步研究。
