Chiu Fu-Chun, Tsai Chin-Feng, Huang Pang-Shuo, Shih Ching-Yu, Tsai Mong-Hsun, Hwang Juey-Jen, Wang Yi-Chih, Chuang Eric Y, Tsai Chia-Ti, Chang Sheng-Nan
Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital Yun-Lin Branch, Dou-Liu City 640, Taiwan.
Division of Cardiology, Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung City 402, Taiwan.
J Clin Med. 2022 Mar 7;11(5):1462. doi: 10.3390/jcm11051462.
Background: Gut microbiome alterations might be considered a metabolic disorder. However, the relationship between the microbiome and acute myocardial infarction (AMI) has not been properly validated. Methods: The feces of 44 subjects (AMI: 19; control: 25) were collected for fecal genomic DNA extraction. The variable region V3−V4 of the 16S rRNA gene was sequenced using the Illumina MiSeq platform. The metabolite amounts were analyzed using the Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathways. Results: The bacteria were more enriched in the AMI group both in the observed operational taxonomic units (OTUs) and faith phylogenetic diversity (PD) (p-value = 0.01 and <0.001 with 95% CI, individually). The Selenomonadales were less enriched in the AMI group at the family, genus, and species levels (all linear discriminant analysis (LDA) scores > 2). Seleno-compounds were more abundant in the AMI group at the family, genus, and species levels (all LDA scores > 2). Conclusions: This is the first study to demonstrate the association of Selenomonadales and seleno-compounds with the occurrence of AMI. Our findings provide an opportunity to identify a novel approach to prevent and treat AMI.
肠道微生物群改变可能被视为一种代谢紊乱。然而，微生物群与急性心肌梗死（AMI）之间的关系尚未得到充分验证。方法：收集44名受试者（AMI组：19例；对照组：25例）的粪便用于提取粪便基因组DNA。使用Illumina MiSeq平台对16S rRNA基因的可变区V3−V4进行测序。使用京都基因与基因组百科全书（KEGG）代谢途径分析代谢物含量。结果：在观察到的操作分类单元（OTU）和置信系统发育多样性（PD）方面，AMI组中的细菌均更为丰富（p值分别为0.01和<0.001，95%置信区间）。在科、属和种水平上，AMI组中月形单胞菌目（Selenomonadales）的富集程度较低（所有线性判别分析（LDA）得分>2）。在科、属和种水平上，AMI组中的硒化合物更为丰富（所有LDA得分>2）。结论：这是第一项证明月形单胞菌目和硒化合物与AMI发生有关的研究。我们的研究结果为确定预防和治疗AMI的新方法提供了机会。
