Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Sede Sureste, Tablaje Catastral No. 31264, km 5.5 Carretera Sierra Papacal-Chuburna Puerto, Interior del Parque Científico y Tecnológico Yucatán, Mérida 97302, Yucatán, Mexico.
Faculty of Science and Technology, Free University of Bozen-Bolzano, Piazza Università 5, 39100 Bolzano, Italy.
Sensors (Basel). 2022 Feb 24;22(5):1794. doi: 10.3390/s22051794.
This work aims to evaluate the purity of chromatographic peaks by a two-dimensional correlation (2D-corr) analysis. Such an analysis leads to two contour plots: synchronous and asynchronous. The synchronous contour plot provides information on the number of peaks present in the chromatogram. The asynchronous contour plot reveals the presence of overlapping species on each peak. The utility of 2D-corr analysis was demonstrated by the chromatographic analysis of chili extracts obtained by HPLC coupled with a coulometric array of sixteen detectors. Thanks to 16 electrochemical sensors, each poised at increasing potentials, the resulting 2D-corr analysis revealed the presence of at least three species on the peak located at a retention time of 0.93 min. Mass spectrometry (MS) analysis was used to analyze the coeluting species, which were identified as: quinic acid (3.593 min), ascorbic acid (3.943 min), and phenylalanine (4.229 min). Overall, this work supports the use of 2D-corr analysis to reveal the presence of overlapping compounds and, thus, verify the signal purity of chromatographic peaks.
本工作旨在通过二维相关（2D-corr）分析评估色谱峰的纯度。这种分析会产生两个等高线图：同步和异步。同步等高线图提供了色谱图中存在的峰数目的信息。异步等高线图揭示了每个峰上存在的重叠物种。通过 HPLC 与十六个电化学传感器阵列耦合得到的辣椒提取物的色谱分析，证明了 2D-corr 分析的实用性。由于 16 个电化学传感器分别位于不同的电位下，得到的 2D-corr 分析揭示了位于保留时间为 0.93 分钟的峰上至少存在三种物质。质谱（MS）分析用于分析共洗脱物质，鉴定为：奎宁酸（3.593 分钟）、抗坏血酸（3.943 分钟）和苯丙氨酸（4.229 分钟）。总的来说，这项工作支持使用 2D-corr 分析来揭示重叠化合物的存在，从而验证色谱峰的信号纯度。
