Food Biotechnology Laboratory, National Center for Genetic Engineering and Biotechnology, Phahonyothin Rd, Pathumthani, 12120, Thailand.
Enzyme Technology Laboratory, National Center for Genetic Engineering and Biotechnology, Phahonyothin Rd, Pathumthani, 12120, Thailand.
Food Microbiol. 2022 Jun;104:103988. doi: 10.1016/j.fm.2022.103988. Epub 2022 Jan 19.
Histamine is a biogenic amine significantly formed in fish sauce leading to a major concern in consumers. This study aimed to identify a halophilic bacterium for histamine degradation in fish sauce, and understand its genomic insight to enhance histamine degradation activity. We discovered the novel halophilic bacterium, Bacillus piscicola FBU1786, degrading histamine and other biogenic amines. Its histamine breakdown was growth-associated in a wide range of NaCl concentrations, pH, and temperature from 4% to 18%, 6.0 to 9.0, and 30 to 45 °C, respectively. Genome sequencing revealed the presence of Cu-binding oxidase-encoding genes and their heterologous expression with Cu supplementation triggered histamine degradation in E. coli. The degree of histamine breakdown in B. piscicola FBU1786 could be enhanced by Cu addition. Histamine degradation of the culture was evaluated in raw fish sauce mixtures to partially mimic the condition during fish sauce fermentation. Histamine degradation was suppressed to the extent of raw fish sauce, but could be restored by Cu supplementation. Together, this study disclosed B. piscicola FBU1786 with the potent histamine degradation activity, identified Cu-binding oxidases responsible for histamine breakdown, and enhanced histamine degradation of the culture using Cu supplementation.
组胺是鱼露中大量形成的生物胺，引起了消费者的极大关注。本研究旨在鉴定一种可用于鱼露中组胺降解的嗜盐细菌，并了解其基因组信息以提高组胺降解活性。我们发现了一种新型的嗜盐细菌，即巴氏鱼球菌 FBU1786，可降解组胺和其他生物胺。其组胺分解与生长相关，在 NaCl 浓度、pH 值和温度范围分别为 4%至 18%、6.0 至 9.0 和 30 至 45°C 时具有广泛的适应性。基因组测序揭示了存在 Cu 结合氧化酶编码基因，并且在补充 Cu 的情况下异源表达这些基因可触发大肠杆菌中的组胺降解。通过添加 Cu 可以增强巴氏鱼球菌 FBU1786 中组胺的分解程度。在原始鱼露混合物中评估了培养物的组胺降解，以部分模拟鱼露发酵过程中的条件。组胺降解受到原始鱼露的抑制，但可通过 Cu 补充得到恢复。总之，本研究揭示了具有强大组胺降解活性的巴氏鱼球菌 FBU1786，鉴定了负责组胺分解的 Cu 结合氧化酶，并通过添加 Cu 来增强培养物的组胺降解。
