解析由环糊精糖基转移酶催化的淀粉外切链长与环糊精生产。
Deciphering external chain length and cyclodextrin production with starch catalyzed by cyclodextrin glycosyltransferase.
机构信息
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, Jiangsu Province, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, Jiangsu Province, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, Jiangsu Province, China.
出版信息
Carbohydr Polym. 2022 May 15;284:119156. doi: 10.1016/j.carbpol.2022.119156. Epub 2022 Jan 20.
Cyclodextrins (CDs) are yielded by cyclodextrin glycosyltransferase (CGTase) using starches or their derivatives as substrates. However, how starch fine structure affects it production is still ambiguous. This study aimed to decipher the relevance between the external chain length (ECL) and CD production. The waxy maize starch was hydrolyzed by β-amylase to prepare starchy substrates with regular gradient ECL for CGTase. The HPAEC results reflected that β-amylolysis lower the starch chain fractions with DP range of 13-24 by 30%, while the fractions with DP < 6 promoted by 30%. The molecular weight distribution and iodine binding results reflected that this process had limited impact on overall starch molecular size parameters. After CGTase conversion, it is interesting to find that the ECL was positively correlated with CD contents, which also has a high consistency among α-CD, β-CD, and γ-CD. This research would provide a favorable perspective for CD production improvement.
环糊精（CDs）是由环糊精葡萄糖基转移酶（CGTase）使用淀粉或其衍生物作为底物生产的。然而，淀粉的精细结构如何影响其生产仍不清楚。本研究旨在阐明外部链长（ECL）与 CD 生产之间的关系。通过β-淀粉酶水解蜡质玉米淀粉，制备具有规则梯度 ECL 的淀粉底物，用于 CGTase。HPAEC 结果表明，β-淀粉酶水解使 DP 范围为 13-24 的淀粉链分数降低了 30%，而 DP<6 的分数则提高了 30%。分子量分布和碘结合结果表明，该过程对淀粉整体分子大小参数的影响有限。在 CGTase 转化后，有趣的是发现 ECL 与 CD 含量呈正相关，其中α-CD、β-CD 和γ-CD 之间也具有高度一致性。本研究为 CD 生产的改进提供了有利的视角。
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