Department of Renal and Genitourinary Surgery, Graduate School of Medical Science, Hokkaido University, Kita 15 Nishi 7, Kita-ku, Sapporo, Hokkaido, 060-8638, Japan.
Sci Rep. 2022 Mar 16;12(1):4540. doi: 10.1038/s41598-022-08612-3.
Parkinson's disease (PD) is a neurodegenerative condition caused by the loss of dopaminergic neurons in the substantia nigra pars compacta. As activation of dopaminergic receptors is fundamentally involved in the micturition reflex in PD, the objective of this study was to determine the effect of a single dose of rotigotine ([-]2-(N-propyl-N-2-thienylethylamino)-5-hydroxytetralin) on intercontraction interval (ICI) and voiding pressure (VP) in a rat model of PD. We used 27 female rats, PD was induced by injecting 6-hydroxydopamine (6-OHDA; 8 μg in 2 μL of 0.9% saline containing 0.3% ascorbic acid), and rotigotine was administrated at doses of 0.125, 0.25, or 0.5 mg/kg, either intravenous or subcutaneous injection. In rats with 6-OHDA-induced PD, intravenous injection of 0.25 or 0.5 mg/kg rotigotine led to a significantly lower ICI than after vehicle injection (p < 0.05). Additionally, VP was significantly lower in animals administered rotigotine compared to those injected with vehicle (p < 0.05). Compared to vehicle-injected animals, subcutaneous administration of rotigotine (0.125, 0.25, or 0.5 mg/kg) led to a significantly higher ICI at 2 h after injection (p < 0.05); however, there was no change in ICI after injection with (+)-SCH23390 hydrochloride. Dermal administration of rotigotine in a rat model of PD could suppress an overactive bladder.
帕金森病（PD）是一种由黑质致密部多巴胺能神经元丧失引起的神经退行性疾病。由于多巴胺受体的激活在 PD 的排尿反射中起着根本作用，因此本研究的目的是确定单剂量罗替戈汀（[-]2-（N-丙基-N-2-噻吩乙基）-5-羟色胺）对帕金森病大鼠模型的间歇收缩间期（ICI）和排尿压（VP）的影响。我们使用了 27 只雌性大鼠，通过注射 6-羟多巴胺（6-OHDA；8μg 溶于 0.9%生理盐水，含 0.3%抗坏血酸）诱导 PD，并以 0.125、0.25 或 0.5mg/kg 的剂量静脉内或皮下给予罗替戈汀。在 6-OHDA 诱导的 PD 大鼠中，静脉内注射 0.25 或 0.5mg/kg 罗替戈汀导致 ICI 明显低于载体注射后（p<0.05）。此外，与给予载体的动物相比，给予罗替戈汀的动物的 VP 明显降低（p<0.05）。与给予载体的动物相比，皮下给予罗替戈汀（0.125、0.25 或 0.5mg/kg）在注射后 2 小时导致 ICI 显著升高（p<0.05）；然而，在注射（+）-SCH23390 盐酸盐后，ICI 没有变化。在 PD 大鼠模型中，皮肤给予罗替戈汀可抑制逼尿肌过度活动。
