5-Heptadecylresorcinol Protects against Atherosclerosis in Apolipoprotein E-Deficient Mice by Modulating SIRT3 Signaling: The Possible Beneficial Effects of Whole Grain Consumption.

SCOPE

Whole grain consumption has been proven to be inversely associated with the risk of cardiovascular diseases. As a biomarker for whole grain dietary intake, 5-heptadecylresorcinol (AR-C17) has attracted increased attention due to its potential health-improving activity. However, the beneficial effect of AR-C17 on atherosclerosis prevention and the underlying mechanism remain unclear.

METHODS AND RESULTS

High-fat diet fed apolipoprotein E-deficient (ApoE ) mice are administrated with or without AR-C17 (30 and 150 mg kg ) for 16 weeks. Histological staining is performed for plaque analysis. Immunofluorescence, western blot, and seahorse cell analysis are carried out to investigate the action of mechanism of AR-C17. The results indicate that AR-C17 supplementation lowered serum total cholesterol, triglyceride, VLDL-C, and LDL-C levels. Moreover, the atherosclerotic plaques in the aortic root region of mice heart are significantly reduced by AR-C17 intervention compared with ApoE control group. In addition, AR-C17 treatment alleviates endothelial cell damage and apoptosis by improving mitochondrial function via sirtuin3 signaling pathway both in ApoE mice and oxidized-LDL-treated human umbilical vein endothelial cells.

CONCLUSION

AR-C17 may be applied as a promising grain-based dietary bioactive ingredient for atherosclerosis prevention. Meanwhile, as a mitochondrial protective agent, it can offer support for the suggested health claim of whole grain diet.