记录自由活动小鼠脑电图痫样放电和行为性癫痫发作的方案。

Protocol for recording epileptiform discharges of EEG and behavioral seizures in freely moving mice.

机构信息

Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Houston, TX 77030, USA.

Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA.

出版信息

STAR Protoc. 2022 Mar 14;3(2):101245. doi: 10.1016/j.xpro.2022.101245. eCollection 2022 Jun 17.

DOI:10.1016/j.xpro.2022.101245

PMID:35310070

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8927982/

Abstract

Recurrent seizures are a common feature in many neurologic disorders. Seizure examination may help with diagnosis, preclinical study, and development of treatment strategies. Here we detail protocols to prepare and implant electrodes, as well as to record and analyze seizure events in freely moving mice. SCA47 mice exhibit both preclinical seizures (i.e., epileptiform discharges of EEG) starting from ∼14 weeks of age and behavioral seizures (i.e., spontaneous behavioral seizures) starting from ∼22 weeks of age. For complete details on the use and execution of this protocol, please refer to Gennarino et al. (2018).

摘要

反复发作的癫痫是许多神经疾病的常见特征。癫痫检查有助于诊断、临床前研究和治疗策略的制定。在这里，我们详细介绍了在自由活动的小鼠中准备和植入电极以及记录和分析癫痫发作事件的方案。SCA47 小鼠从 14 周龄左右开始出现临床前癫痫（即 EEG 的癫痫样放电），从 22 周龄左右开始出现行为性癫痫（即自发性行为性癫痫）。有关本协议的使用和执行的完整详细信息，请参阅 Gennarino 等人。（2018 年）。