Huang Qi, Yang Zhi, Tian Jin-Hai, You Pei-Dong, Wang Jia, Ma Rong, Yu Jingjing, Zhang Xu, Cao Jia, Cao Jie, Wang Li-Bin
The Biochip Research Center of General Hospital of Ningxia Medical University, Yinchuan 750004, China.
Guangzhou First People's Hospital, Guangzhou 510180, China.
J Oncol. 2022 Mar 11;2022:4181730. doi: 10.1155/2022/4181730. eCollection 2022.
To investigate the effects of LncRNA SNHG1 on the proliferation, migration, and epithelial-mesenchymal transition (EMT) of colorectal cancer cells (CRCs).
4 pairs of CRC tissue samples and their corresponding adjacent samples were analyzed by the human LncRNA microarray chip. The expression of LncSNHG1 in CRC cell lines was verified by qRT-PCR. Colony formation assays and CCK8 assays were applied to study the changes in cell proliferation. The transwell assay and wound healing experiments were used to verify the cell invasion and migration. EMT progression was confirmed finally.
LncSNHG1 was overexpressed both in CRC tissues and cell lines, while the miR-181b-5p expression was decreased in CRC cell lines. After knock-down of LncSNHG1, the proliferation, invasion, and migration of HT29 and SW620 cells were all decreased. Meanwhile, LncSNHG1 enhanced EMT progress through regulation of the miR-181b-5p/SMAD2 axis.
LncSNHG1 promotes colorectal cancer cell proliferation and invasion through the miR-181b-5p/SMAD2 axis.
探讨长链非编码RNA SNHG1对结肠癌细胞（CRC）增殖、迁移及上皮-间质转化（EMT）的影响。
采用人长链非编码RNA微阵列芯片分析4对CRC组织样本及其相应的癌旁样本。通过qRT-PCR验证LncSNHG1在CRC细胞系中的表达。应用集落形成试验和CCK8试验研究细胞增殖的变化。采用Transwell试验和伤口愈合实验验证细胞侵袭和迁移能力。最终确认EMT进程。
LncSNHG1在CRC组织和细胞系中均高表达，而miR-181b-5p在CRC细胞系中的表达降低。敲低LncSNHG1后，HT29和SW620细胞的增殖、侵袭和迁移能力均下降。同时，LncSNHG1通过调控miR-181b-5p/SMAD2轴增强EMT进程。
LncSNHG1通过miR-181b-5p/SMAD2轴促进结肠癌细胞增殖和侵袭。
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