外周血T细胞亚群与甲状腺相关性眼病活动度的相关性
Correlation between Peripheral T Cell Subsets and the Activity of Thyroid-Associated Ophthalmopathy.
作者信息
Hu Hong, Liang Liang, Ge Qian, Jiang Xing, Fu Zhizheng, Liu Chun, Long Jian
机构信息
Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Chongqing Key Laboratory of Ophthalmology, and Chongqing Eye Institute, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
出版信息
Int J Endocrinol. 2022 Mar 8;2022:2705650. doi: 10.1155/2022/2705650. eCollection 2022.
INTRODUCTION
Thyroid-associated ophthalmopathy (TAO) is the most common orbital immunological disease in adults. T cells play an important role in the pathogenesis of TAO. However, our knowledge of the circulating T cell subsets in TAO is limited.
OBJECTIVE
To investigate the circulating T cell subsets in TAO and the correlations between them and the activity of TAO.
METHODS
Thirty-eight TAO patients (19 active and 19 nonactive) were enrolled. The absolute number and percentage of total lymphocytes, CD3+T cells, CD4+T cells, CD8+ T cells, CD3+CD4-CD8-T cells (DNT cells), and CD3+CD4+CD8+ T cells (DPT cells) in peripheral blood were measured by flow cytometer.
RESULTS
TAO patients were divided into the active group and the nonactive group by the clinical activity score (CAS). The mean CAS was 4 ± 1.11 in the active group and 1.47 ± 0.61 in the nonactive group. No statistical differences were found in gender, age, and the levels of FT3, FT4, TSH, and TRAb between the two groups. The percentage of DNT cells was lower in the active group than in the non-active group, and it was negatively correlated with CAS ( = -0.349, =0.032), but not the absolute number. The CD4/CD8 ratio, the absolute number and percentage of CD3+ T cells, CD4+ T cells, CD8+ T cells, and DPT cells did not differ between the two groups.
CONCLUSION
In the present study, we found the percentage of DNT cells was significantly lesser in the active TAO than in the nonactive TAO, and it was negatively correlated with the activity of the TAO. It suggests that DNT cells may involve in the immunopathogenesis of TAO and can serve as a clinical biomarker of the disease activity.
引言
甲状腺相关眼病（TAO）是成人中最常见的眼眶免疫性疾病。T细胞在TAO的发病机制中起重要作用。然而，我们对TAO中循环T细胞亚群的了解有限。
目的
研究TAO中循环T细胞亚群及其与TAO活动度之间的相关性。
方法
纳入38例TAO患者（19例活动期和19例非活动期）。采用流式细胞仪检测外周血中淋巴细胞总数、CD3⁺T细胞、CD4⁺T细胞、CD8⁺T细胞、CD3⁺CD4⁻CD8⁻T细胞（双阴性T细胞，DNT细胞）和CD3⁺CD4⁺CD8⁺T细胞（双阳性T细胞，DPT细胞）的绝对数量和百分比。
结果
根据临床活动评分（CAS）将TAO患者分为活动组和非活动组。活动组的平均CAS为4±1.11，非活动组为1.47±0.61。两组在性别、年龄以及FT3、FT4、TSH和TRAb水平方面均未发现统计学差异。活动组中DNT细胞的百分比低于非活动组，且与CAS呈负相关（r=-0.349，P=0.032），但绝对数量无相关性。两组之间CD4/CD8比值、CD3⁺T细胞、CD4⁺T细胞、CD8⁺T细胞和DPT细胞的绝对数量和百分比无差异。
结论
在本研究中，我们发现活动期TAO中DNT细胞的百分比明显低于非活动期TAO，且与TAO的活动度呈负相关。这表明DNT细胞可能参与TAO的免疫发病机制，并可作为该疾病活动度的临床生物标志物。
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