Laboratory of Biology and Physiology of Organisms (LBPO), Biological Sciences Faculty, Houari Boumediène Sciences and Technology University (USTHB), BP 32, El-Alia, Bab Ezzouar, Algiers 16111, Algeria.
Laboratory of Molecular Biology, Cluster of Plant Developmental Biology, Plant Science Group, Wageningen University and Research (WUR), Droevendaalsesteeg 1, Wageningen 6708PB, The Netherlands.
Mol Plant Microbe Interact. 2022 Jul;35(7):592-603. doi: 10.1094/MPMI-12-21-0294-FI. Epub 2022 Jun 17.
Plants harbor in and at their roots bacterial microbiomes that contribute to their health and fitness. The microbiome composition is controlled by the environment and plant genotype. Previously, it was shown that the plant genotype-dependent dissimilarity of root microbiome composition of different species becomes smaller under drought stress. However, it remains unknown whether this reduced plant genotype-dependent effect is a specific response to drought stress or a more generic response to abiotic stress. To test this, we studied the effect of salt stress on two distinct barley ( L.) genotypes: the reference cultivar Golden Promise and the Algerian landrace AB. As inoculum, we used soil from salinized and degraded farmland on which barley was cultivated. Controlled laboratory experiments showed that plants inoculated with this soil displayed growth stimulation under high salt stress (200 mM) in a plant genotype-independent manner, whereas the landrace AB also showed significant growth stimulation at low salt concentrations. Subsequent analysis of the root microbiomes revealed a reduced dissimilarity of the bacterial communities of the two barley genotypes in response to high salt, especially in the endophytic compartment. High salt level did not reduce α-diversity (richness) in the endophytic compartment of both plant genotypes but was associated with an increased number of shared strains that respond positively to high salt. Among these, spp. were most abundant. These findings suggest that the plant genotype-dependent microbiome composition is altered generically by abiotic stress.[Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
植物在其根部携带有细菌微生物组，这些微生物组有助于植物的健康和活力。微生物组的组成受环境和植物基因型的控制。以前的研究表明，不同物种的根微生物组组成在干旱胁迫下，植物基因型依赖性的差异变小。然而，目前尚不清楚这种减少的植物基因型依赖性效应是对干旱胁迫的特异性反应，还是对非生物胁迫的更普遍反应。为了验证这一点，我们研究了盐胁迫对两种不同的大麦（L.）基因型的影响：参考品种 Golden Promise 和阿尔及利亚地方品种 AB。作为接种物，我们使用了在盐化和退化农田上种植大麦的土壤。受控实验室实验表明，用这种土壤接种的植物在高盐胁迫（200 mM）下以植物基因型独立的方式表现出生长刺激，而地方品种 AB 也在低盐浓度下表现出显著的生长刺激。随后对根微生物组的分析表明，两种大麦基因型对高盐的细菌群落的相似性降低，尤其是在根内共生区。高盐水平并没有降低两种植物基因型根内共生区的 α-多样性（丰富度），但与对高盐反应积极的共享菌株数量增加有关。其中， 种最为丰富。这些发现表明，非生物胁迫通常会改变植物基因型依赖性的微生物组组成。[公式：见文本]版权所有 2022 年作者。这是一个在 CC BY-NC-ND 4.0 国际许可下分发的开放获取文章。
