Jhan Pijush Kanti, Lee Kyeong-Yeoll
Division of Applied Biosciences, College of Agriculture and Life Science, Kyungpook National University, Daegu, Republic of Korea.
Department of Agriculture, Noakhali Science and Technology University, Noakhali, Bangladesh.
Arch Insect Biochem Physiol. 2022 Jun;110(2):e21890. doi: 10.1002/arch.21890. Epub 2022 Mar 23.
Bemisia tabaci Mediterranean (MED) is a highly invasive cryptic species complex found in the world's tropical, subtropical, and temperate regions. It is a severe pest of various crops and a vector of plant pathogenic viruses, particularly geminiviruses. Thermal acclimation of insects is a critical for the survival in unfavorable temperature condition. We observed that great survival rate of B. tabaci MED at the uncontrolled greenhouse which had fluctuating temperature condition (FTC) from 10°C to 60°C in spring and summer season. Our study showed that while B. tabaci MED reared under FTC for 10 weeks from April to June, its survival rate was gradually increased when heat shock was treated 50°C for 0.5 h. In contrast, the same heat shock treatment was lethal in the colony reared under constant temperature condition (CTC) at the controlled insectary. After being acclimated, the lethal temperatures LT , LT , and LT under CTC were 47.7°C, 50.1°C, and 50.3°C, whereas those under FTC were 59.8°C, 62.7°C, and 63.0°C, respectively. In addition, we observed that the transcript levels of three investigated heat shock protein (HSP) genes (hsp20, hsp70, and hsp90) were lower under FTC than under CTC. This study suggests that B. tabaci MED retains high heat acclimation ability, making it tolerant of extreme thermal conditions.
烟粉虱地中海隐种（MED）是一种极具入侵性的复合隐性物种，分布于世界热带、亚热带和温带地区。它是多种作物的严重害虫，也是植物致病病毒特别是双生病毒的传播媒介。昆虫的热适应对于在不利温度条件下的生存至关重要。我们观察到，在春季和夏季温度在10°C至60°C波动的非控制温室中，烟粉虱地中海隐种具有很高的存活率。我们的研究表明，从4月到6月，烟粉虱地中海隐种在波动温度条件下饲养10周，当在50°C下热激处理0.5小时时，其存活率逐渐增加。相比之下，在可控昆虫饲养室的恒温条件下饲养的群体中，相同的热激处理是致命的。适应后，恒温条件下的致死温度LT₅₀、LT₉₀和LT₉₅分别为47.7°C、50.1°C和50.3°C，而波动温度条件下的分别为59.8°C、62.7°C和63.0°C。此外，我们观察到，在波动温度条件下，三个被研究的热休克蛋白（HSP）基因（hsp20、hsp70和hsp90）的转录水平低于恒温条件下。这项研究表明，烟粉虱地中海隐种保留了很高的热适应能力，使其能够耐受极端热条件。
