基于大麦条纹花叶病毒（BSMV）的病毒诱导基因沉默技术用于小麦和大麦基因的功能表征。

Barley Stripe Mosaic Virus (BSMV)-Based Virus-Induced Gene Silencing to Functionally Characterize Genes in Wheat and Barley.

机构信息

Centre for Plant Health, Canadian Food Inspection Agency, North Saanich, BC, Canada.

Department of Plant Pathology, North Dakota State University, Fargo, ND, USA.

出版信息

Methods Mol Biol. 2022;2408:85-93. doi: 10.1007/978-1-0716-1875-2_5.

DOI:10.1007/978-1-0716-1875-2_5

PMID:35325417

Abstract

Virus-induced gene silencing (VIGS) is an efficient method for functional characterization of genes in monocot and dicot plants via transient silencing of gene(s) of interest. Among various virus vectors, Barley stripe mosaic virus (BSMV) is established as a vector of choice to silence genes in wheat and barley. BSMV is a single-stranded positive-sense RNA virus with a tripartite genome consisting of α, β, and γ RNAs. BSMV-based VIGS has been used to silence both abiotic and biotic stress response genes in various growth stages of plants. Here we describe an efficient and effective protocol to successfully silence wheat and barley genes expressing in various tissues using this approach.

摘要

病毒诱导的基因沉默（VIGS）是一种通过瞬时沉默感兴趣的基因来研究单子叶和双子叶植物中基因功能的有效方法。在各种病毒载体中，大麦黄花叶病毒（BSMV）已被确立为在小麦和大麦中沉默基因的首选载体。BSMV 是一种单链正链 RNA 病毒，具有由 α、β 和 γ RNA 组成的三分体基因组。基于 BSMV 的 VIGS 已被用于在植物的各种生长阶段沉默非生物和生物胁迫反应基因。在这里，我们描述了一种使用该方法在各种组织中成功沉默小麦和大麦基因的有效且有效的方案。