Medolac Laboratories, A Public Benefit Corporation, Department of Neonatal Immunology and Microbiology, Boulder City, Nevada, USA.
Breastfeed Med. 2022 Jun;17(6):501-505. doi: 10.1089/bfm.2021.0242. Epub 2022 Mar 21.
Human milk antibodies specific to allergen enhance immunological tolerance in neonates by educating their immature mucosal immunity. The impact of restricting food allergens in diet and maternal factors on the levels of allergen-specific antibodies in human milk remains unclear. We aimed to evaluate the influence of the maternal avoidance diet of cow's milk on the titers of IgG, SIgA/IgA, and IgM specific to β-lactoglobulin (BLG) in human milk. Human milk samples were collected from 26 women nonrestricting cow's milk and 11 women restricting cow's milk. The titers of IgG, SIgA/IgA, and SIgM/IgM specific to BLG were measured using ELISA. BLG-specific IgG titers were 2.9-fold higher in women nonrestricting cow's milk than those restricting cow's milk in their diet ( = 0.026), but BLG-specific SIgA/IgA and SIgM/IgM titers were comparable between these two groups. BLG-specific IgG was positively correlated with BLG-specific SIgA/IgA titers in milk from mothers nonrestricting cow's milk ( = 0.0007) but did not correlate for mothers restricting cow's milk. BLG-specific SIgA/IgA titer decreased with increasing postpartum time in milk from women restricting cow's milk ( = 0.019). Type of delivery, infant gender, maternal age, and probiotic intake did not influence the BLG-specific antibody titers. This study reveals that the secretion of BLG-specific IgG in human milk increases in women nonrestricting cow's milk compared with women restricting cow's milk. The role of breast milk allergen-specific antibodies on the neonatal gut (crosstalk with immune and epithelial cells) remains to be investigated.
人乳中的过敏原特异性抗体通过教育其不成熟的黏膜免疫来增强新生儿的免疫耐受性。饮食中限制食物过敏原和母体因素对人乳中过敏原特异性抗体水平的影响尚不清楚。我们旨在评估母亲避免食用牛奶对人乳中β-乳球蛋白（BLG）特异性 IgG、SIgA/IgA 和 IgM 滴度的影响。从 26 名非限制牛奶饮食的女性和 11 名限制牛奶饮食的女性中收集人乳样本。使用 ELISA 测量 BLG 特异性 IgG、SIgA/IgA 和 SIgM/IgM 滴度。与限制饮食中的女性相比，非限制牛奶饮食的女性的 BLG 特异性 IgG 滴度高 2.9 倍（=0.026），但两组的 BLG 特异性 SIgA/IgA 和 SIgM/IgM 滴度相当。非限制牛奶饮食的母亲的人乳中 BLG 特异性 IgG 与 BLG 特异性 SIgA/IgA 滴度呈正相关（=0.0007），但限制牛奶饮食的母亲则没有相关性。限制牛奶饮食的女性人乳中的 BLG 特异性 SIgA/IgA 滴度随产后时间的增加而降低（=0.019）。分娩方式、婴儿性别、母亲年龄和益生菌摄入均不影响 BLG 特异性抗体滴度。本研究表明，与限制牛奶饮食的女性相比，非限制牛奶饮食的女性人乳中 BLG 特异性 IgG 的分泌增加。人乳过敏原特异性抗体在新生儿肠道中的作用（与免疫和上皮细胞的相互作用）仍有待研究。
