Majewska Małgorzata P, Miltko Renata, Bełżecki Grzegorz, Kędzierska Aneta, Kowalik Barbara
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland.
Animals (Basel). 2022 Mar 10;12(6):699. doi: 10.3390/ani12060699.
The aim of the study was to compare two sources of tannins on fatty acids (FA) composition in rumen. Treatments were (g tannins/kg diet as-feed-basis) as follows: (1) no supplemental tannin addition (CON), (2) addition of 13 g of oak bark extract (OAK), and (3) 4 g of tannic acid (TAN). The basal diet contained 55:45 forage to concentrate ratio. Net consumption of tannins (g/d) was 4 g for both tannins sources. The study was performed on three Polish Mountain ewes fitted with rumen cannulas, and was divided into three experimental periods (I, II, and III). Both sampling time and animal diet had a significant effect on FA profile in the rumen fluid. In general, FA concentrations were higher before feeding in comparison to samples collected 2 and 4 h after feeding. In terms of dietary effect, it was shown that TAN addition had a greater influence on FA profile in the ruminal fluid than the OAK diet. Briefly, in the TAN group significantly increased concentrations of C18:2 (linoleic acid, LA) 8 h after feeding (vs. control, CON and OAK), C18:3 (α-linolenic acid, LNA) 4 h after feeding (vs. OAK), C20:3 n-6 before feeding (vs. CON), C20:4 before feeding (vs. CON and OAK) and 8 h after feeding (vs. OAK) were recorded. In contrast, OAK addition significantly reduced C20:3 n-6 concentration 2 h after feeding (vs. CON). In conclusion, increased concentrations of both LA and LNA in the rumen indicated that supplemental tannic acid may inhibit the initial stage of FA biohydrogenation in the rumen.
本研究的目的是比较两种单宁来源对瘤胃中脂肪酸（FA）组成的影响。处理方式（以每千克日粮干物质计的单宁克数）如下：（1）不添加额外单宁（CON），（2）添加13克橡树皮提取物（OAK），（3）添加4克单宁酸（TAN）。基础日粮的粗饲料与精饲料比例为55:45。两种单宁来源的单宁净摄入量（克/天）均为4克。本研究选用三只安装了瘤胃瘘管的波兰山地母羊进行，分为三个实验期（I、II和III）。采样时间和动物日粮对瘤胃液中的FA谱均有显著影响。一般来说，与采食后2小时和4小时采集的样本相比，采食前的FA浓度更高。在日粮影响方面，结果表明，添加TAN对瘤胃液中FA谱的影响大于OAK日粮。简而言之，在TAN组中，采食后8小时C18:2（亚油酸，LA）浓度显著升高（与对照组CON和OAK相比），采食后4小时C18:3（α-亚麻酸，LNA）浓度显著升高（与OAK相比），采食前C20:3 n-6浓度显著升高（与CON相比），采食前和采食后8小时C20:4浓度显著升高（与CON和OAK相比）。相比之下，添加OAK显著降低了采食后2小时C20:3 n-6的浓度（与CON相比）。总之，瘤胃中LA和LNA浓度的增加表明，补充单宁酸可能会抑制瘤胃中FA生物氢化的初始阶段。
