Vingolo Enzo M, Casillo Lorenzo, Contento Laura, Toja Francesca, Florido Antonio
Department of Biotechnology and Medical-Surgical Sciences, "Sapienza" University of Rome, 04100 Latina, Italy.
Biomedicines. 2022 Mar 2;10(3):582. doi: 10.3390/biomedicines10030582.
Purpose: Retinitis Pigmentosa is a term that includes a group of inherited bilateral and progressive retinal degenerations, with the involvement of rod photoreceptors, which frequently leads to blindness; oxidative stress may be involved in the degeneration progression as proposed by several recent studies. The goal of this study is to evaluate whether circulating free radicals taken from capillary blood are related to one of the most important features of Retinitis pigmentosa that can affect frequently patients: cystoid macular oedema (CME). Materials: A total of 186 patients with Retinitis Pigmentosa (range: 25−69 years) were enrolled; all patients completed an ophthalmologic examination and SD-OCT at baseline and were divided into three subgroups according to the SD-OCT features. ROS blood levels were determined using FORT with monitoring of free oxygen radicals. Results: Test levels of free oxygen radicals were significantly increased, almost twice, in RP patients showing cystoid macular oedema and significantly increased compared to the control group. (p < 0.001). Discussion: Our findings suggest that oxidative stress may speed cone photoreceptors’ morphological damage (CMT); because long lasting oxidative stress in the RP may cause oxidative damage, with animal models of RP suggesting this is a micromolecular mechanism of photoreceptors’ (cone) death, it can be similar to cone damage in human RP eyes. The limitations of this paper are the relatively small sample, the horizontal design of the study, and the lack of data about the levels of ROS in the vitreous body.
视网膜色素变性是一个术语，涵盖了一组遗传性双侧进行性视网膜变性，累及视杆光感受器，常导致失明；如最近几项研究所提出的，氧化应激可能参与了变性的进展。本研究的目的是评估从毛细血管血中获取的循环自由基是否与视网膜色素变性最重要的特征之一（可频繁影响患者）：黄斑囊样水肿（CME）相关。材料：共纳入186例视网膜色素变性患者（年龄范围：25 - 69岁）；所有患者在基线时完成了眼科检查和SD - OCT，并根据SD - OCT特征分为三个亚组。使用FORT测定ROS血液水平并监测游离氧自由基。结果：在出现黄斑囊样水肿的视网膜色素变性患者中，游离氧自由基的检测水平显著升高，几乎是对照组的两倍，且与对照组相比显著升高（p < 0.001）。讨论：我们的研究结果表明，氧化应激可能加速视锥光感受器的形态损伤（CMT）；因为视网膜色素变性中持久的氧化应激可能导致氧化损伤，视网膜色素变性动物模型表明这是光感受器（视锥）死亡的一种微观分子机制，这可能与人类视网膜色素变性眼中的视锥损伤相似。本文的局限性在于样本相对较小、研究为横向设计以及缺乏玻璃体中ROS水平的数据。
